Tian Tian, Cai You, Qin Xin, Wang Jiangang, Wang Yali, Yang Xin
Shenzhen Key Laboratory of Translational Research for Brain Diseases, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Department of Neurology, Shenzhen Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Front Cell Neurosci. 2023 Feb 24;17:1114037. doi: 10.3389/fncel.2023.1114037. eCollection 2023.
Forebrain neural networks are vital for cognitive functioning, and their excitatory-inhibitory (E-I) balance is governed by neural homeostasis. However, the homeostatic control strategies and transcriptomic mechanisms that maintain forebrain E-I balance and optimal cognition remain unclear.
We used patch-clamp and RNA sequencing to investigate the patterns of neural network homeostasis with suppressing forebrain excitatory neural activity and spatial training.
We found that inhibitory transmission and receptor transcription were reduced in tamoxifen-inducible Kir2.1 conditional knock-in mice. In contrast, spatial training increased inhibitory synaptic connections and the transcription of inhibitory receptors.
Our study provides significant evidence that inhibitory systems play a critical role in the homeostatic control of the E-I balance in the forebrain during cognitive training and E-I rebalance, and we have provided insights into multiple gene candidates for cognition-related homeostasis in the forebrain.
前脑神经网络对认知功能至关重要，其兴奋-抑制（E-I）平衡受神经稳态调控。然而，维持前脑E-I平衡和最佳认知的稳态控制策略及转录组机制仍不清楚。
我们使用膜片钳和RNA测序来研究通过抑制前脑兴奋性神经活动和空间训练实现神经网络稳态的模式。
我们发现，在他莫昔芬诱导的Kir2.1条件性敲入小鼠中，抑制性传递和受体转录减少。相反，空间训练增加了抑制性突触连接和抑制性受体的转录。
我们的研究提供了重要证据，表明抑制性系统在认知训练和E-I重新平衡过程中对前脑E-I平衡的稳态控制起关键作用，并且我们对前脑中与认知相关的稳态的多个基因候选物有了深入了解。
