Identification and enzymatic activity analysis of the gene family associated with glucosinolate biosynthesis in Tumorous stem mustard ( var. ).

The major enzyme encoded by the glucosinolate biosynthetic gene is involved in catalyzing the conversion of glucoiberin (GIB) into sinigrin (SIN) in Brassicaceae crops. The proteins have previously been identified in several Brassicaceae species, but not in Tumorous stem mustard. As per this research, the five identified members of the family from the whole genome of named - were found to be evenly distributed on five chromosomes. The subcellular localization results implied that BjuAOP2 proteins were mainly concentrated in the cytoplasm. Phylogenetic analysis of the AOP2 proteins from the sequenced species in BRAD showed that genes were more closely linked to and than . In comparison with other plants, the members were conserved in terms of gene structures, protein sequences, and motifs. The light response and hormone response elements were included in the genes' cis-regulatory elements. The expression pattern of genes was influenced by the different stages of development and the type of tissue being examined. The BjuAOP2 proteins were used to perform the heterologous expression experiment. The results showed that all the five BjuAOP2 proteins can catalyze the conversion of GIB to SIN with different catalytic activity. These results provide the basis for further investigation of the functional study of in Tumorous stem mustard glucosinolate biosynthesis.