Wang Ran, Zhang Qinghe, Qu Cheng, Wang Qian, Wang Jinda, Luo Chen
Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
National Engineering Research Center for Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, China.
Front Plant Sci. 2023 Feb 22;14:1150853. doi: 10.3389/fpls.2023.1150853. eCollection 2023.
is a threat to agriculture worldwide because of its potential to cause devastating damage to crops. Chlorogenic acid is a bioactive pesticidal phytochemical agent against various insect pests. We here determined the susceptibility of a laboratory strain of to chlorogenic acid and other popular insecticides, and the susceptibility of several field-collected populations to chlorogenic acid. Also, cross-resistance to four common insecticides was measured. Chlorogenic acid had the highest toxicity of all tested insecticides, and all the field-collected populations were susceptible to chlorogenic acid, and little cross-resistance was detected between chlorogenic acid and the other tested insecticides. Furthermore, analysis of enzyme activities and expression of P450 genes in after treatment with LC of chlorogenic acid suggested that enhanced P450 activity could be involved in chlorogenic acid detoxification. We subsequently evaluated sublethal effects of chlorogenic acid, and found that treatment with LC of chlorogenic acid prolonged duration of two developmental stages, reduced fecundity, and decreased survival rates of treated compared to untreated insects. Overall, these findings demonstrate strong toxicity and significant sublethal effects of chlorogenic acid on , and suggest that overexpression of P450 genes may be associated with chlorogenic acid detoxification.
由于其有可能对农作物造成毁灭性损害，因此对全球农业构成威胁。绿原酸是一种针对多种害虫的具有生物活性的杀虫植物化学药剂。我们在此测定了一种实验室品系对绿原酸和其他常用杀虫剂的敏感性，以及几个野外采集种群对绿原酸的敏感性。此外，还测定了对四种常见杀虫剂的交叉抗性。绿原酸在所有测试的杀虫剂中具有最高的毒性，并且所有野外采集的种群对绿原酸敏感，在绿原酸与其他测试杀虫剂之间几乎未检测到交叉抗性。此外，在用绿原酸的半数致死浓度处理后对其体内酶活性和P450基因表达的分析表明，增强的P450活性可能参与绿原酸的解毒过程。我们随后评估了绿原酸的亚致死效应，发现用绿原酸的半数致死浓度处理会延长两个发育阶段的持续时间，降低繁殖力，并与未处理的昆虫相比降低处理后的昆虫的存活率。总体而言，这些发现证明了绿原酸对[昆虫名称未给出]具有很强的毒性和显著的亚致死效应，并表明P450基因的过表达可能与绿原酸解毒有关。
