Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, China.
J Cell Physiol. 2024 Oct;239(10):e30888. doi: 10.1002/jcp.30888. Epub 2022 Oct 2.
Botanical insecticides are the origin of all insecticidal compounds. They have been widely used to control pests in crops for a long time. Currently, the commercial production of botanical insecticides extracted from plants is limited because of insufficient raw material supply. Synthetic biology is a promising and effective approach for addressing the current problems of the production of botanical insecticides. It is an emerging biological research hotspot in the field of botanical insecticides. However, the biosynthetic pathways of many botanical insecticides are not completely elucidated. On the other hand, the cytotoxicity of botanical pesticides and low efficiency of these biosynthetic enzymes in new hosts make it still challenging for their heterologous production. In the present review, we summarized the recent developments in the heterologous production of botanical insecticides, analyzed the current challenges, and discussed the feasible production strategies, focusing on elucidating biosynthetic pathways, enzyme engineering, host engineering, and cytotoxicity engineering. Looking to the future, synthetic biology promises to further advance heterologous production of more botanical pesticides.
植物源杀虫剂是所有杀虫剂化合物的起源。它们长期以来一直被广泛用于控制农作物害虫。目前，由于原料供应不足，植物源杀虫剂的商业生产受到限制。合成生物学是解决目前植物源杀虫剂生产问题的一种有前途和有效的方法。它是植物源杀虫剂领域新兴的生物研究热点。然而，许多植物源杀虫剂的生物合成途径尚未完全阐明。另一方面，植物源农药的细胞毒性和这些生物合成酶在新宿主中的低效率使得它们的异源生产仍然具有挑战性。在本综述中，我们总结了植物源杀虫剂异源生产的最新进展，分析了当前的挑战，并讨论了可行的生产策略，重点是阐明生物合成途径、酶工程、宿主工程和细胞毒性工程。展望未来，合成生物学有望进一步推进更多植物源农药的异源生产。
