Yashvardhini Niti, Jha Deepak Kumar, Kumar Amit, Gaurav Manjush, Sayrav Kumar
Department of Microbiology, Patna Women's College, Patna, 800 001, Bihar, India.
Department of Zoology, S.M.P. Girls Degree College, Ballia, 277401, Uttar Pradesh, India.
Bioinformation. 2022 Apr 30;18(4):432-437. doi: 10.6026/97320630018432. eCollection 2022.
SARS-CoV-2 (Severe Acute Respiratory Syndrome), a causative agent of COVID-19 disease created a pandemic situation worldwide. Nsp15 is a uridine specific endoribonuclease encoded by the genome of SARS-CoV-2. It plays important role in processing viral RNA and, thus evades the host immune system. Therefore, it is of interest to identify mutants of nsp15 amongst Asian SARS-CoV-2 isolates, where a total of 1795 mutations, from 7793 sequences of Asia submitted till 31st January 2022, amongst which A231V, H234Y, K109N, K259R and S261A mutations were found frequent. Hence, we report data on the predicted secondary structure of wild type form followed by hydropathy plot, physiochemical properties, Ramachandran plot, B-cell epitopes prediction and protein modeling of wild type and mutant of nsp15 protein. Data shows that nsp15 of SARS-CoV-2 is a pontential candidate for the development of vaccine to control the infections of SARS-CoV-2.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是导致新型冠状病毒肺炎（COVID-19）疾病的病原体，在全球造成了大流行态势。Nsp15是一种由SARS-CoV-2基因组编码的尿苷特异性核糖核酸内切酶。它在处理病毒RNA过程中发挥重要作用，从而逃避宿主免疫系统。因此，在亚洲SARS-CoV-2分离株中鉴定nsp15的突变体具有重要意义，截至2022年1月31日提交的来自亚洲的7793个序列中共有1795个突变，其中A231V、H234Y、K109N、K259R和S261A突变较为常见。因此，我们报告了野生型nsp15蛋白的预测二级结构数据，随后是亲水性图谱、理化性质、拉氏图、B细胞表位预测以及野生型和突变型nsp15蛋白的蛋白质建模。数据表明，SARS-CoV-2的nsp15是开发控制SARS-CoV-2感染疫苗的潜在候选对象。
