Nan Yi, Liang Haizhen, Pang Xu, Zheng Wei, Shi Yuhao, Chen Xiaojuan, Zhang Jie, Song Juan, Ma Baiping
Beijing Institute of Radiation Medicine, Beijing, China.
Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Front Chem. 2023 Feb 23;11:1146153. doi: 10.3389/fchem.2023.1146153. eCollection 2023.
The rhizoma of (PO) is used to treat yin injuries of the lung and stomach in traditional Chinese medicine. The chemical constituents of this herb are steroidal saponins, homoisoflavanones, and alkaloids. Xiangyuzhu (XPO) and Guanyuzhu (GPO) are available in the market as two specifications of the commodity. Nonetheless, systematic research on the identification and comparison of chemical constituents of these two commercial specifications is yet lacking. Herein, an integrated method combing ultra-high-performance liquid chromatography-quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) with ultra-high-performance liquid chromatography-charged aerosol detection (UHPLC-CAD) was employed for the comprehensively qualitative and quantitative analyses of PO. A total of 62 compounds were identified by UHPLC-Q-TOF/MS, among which 13 potential chemical markers were screened out to distinguish two commercial specifications. Subsequently, the absolute determination method for polygodoraside G, polygonatumoside F, and timosaponin H1 was established and validated by UHPLC-CAD. The contents of the three compounds were 13.33-236.24 μg/g, 50.55-545.04 μg/g, and 13.34-407.83 μg/g, respectively. Furthermore, the ratio of timosaponin H1/polygodoraside G could be applied to differentiate the two specifications. Samples with a ratio <2 are considered XPO and >5 are considered GPO. Therefore, the above results provide a valuable means for the quality control of PO.
玉竹的根茎在传统中药中用于治疗肺胃阴虚。该草药的化学成分包括甾体皂苷、高异黄酮和生物碱。湘玉竹（XPO）和关玉竹（GPO）作为该商品的两种规格在市场上有售。然而，目前仍缺乏对这两种商业规格化学成分的鉴定和比较的系统研究。在此，采用超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOF/MS）与超高效液相色谱- charged气溶胶检测（UHPLC-CAD）相结合的综合方法对玉竹进行全面的定性和定量分析。通过UHPLC-Q-TOF/MS共鉴定出62种化合物，其中筛选出13种潜在的化学标志物以区分两种商业规格。随后，建立了玉竹苷G、玉竹苷F和知母皂苷H1的绝对定量方法，并通过UHPLC-CAD进行了验证。这三种化合物的含量分别为13.33 - 236.24μg/g、50.55 - 545.04μg/g和13.34 - 407.83μg/g。此外，知母皂苷H与玉竹苷G的比值可用于区分这两种规格。比值<2的样品被认为是XPO，>5的样品被认为是GPO。因此，上述结果为玉竹的质量控制提供了有价值的手段。
