Afolabi Olajide Joseph, Oluwafemi Olubanke Rosena, Oniya Mobolanle Oladipo
Parasitology and Public Health Unit, Department of Biology, Federal University of Technology, Akure, Ondo State Nigeria.
Department of Pediatrics, Mother and Child Hospital, Akure, Ondo State Nigeria.
J Parasit Dis. 2023 Mar;47(1):59-67. doi: 10.1007/s12639-022-01538-7. Epub 2022 Oct 17.
Malaria parasite resistant to drugs has been a major barrier to effective treatment of malaria. Therefore, the study aimed to evaluate the distribution resistant Kelch protein gene on chromosome 13 (Kelch 13) and multidrug resistant () mutant genes among children aged five years and below who attended Mother and Child Hospital, Akure, Nigeria. Thin and thick smears were prepared from the blood collected aseptically through venepuncture from five hundred (500) children. Structured questionnaires were used to obtain demographic data from the respondents. Two hundred malaria positive samples were randomly selected from the 500 samples for PCR analysis to detect and mutant genes. The results showed that of the 500 respondents, 288 (57.6%) were males while 21 (42.4%) were females. Pfmdr1distribution include: mixed group (mutant/wild) 38.5%, mutant gene 35.5%, wild gene 20.5% and the resistant genes were absent in 5.5% of the infected children. The mixed group of gene was higher among infants (51.9%), children with birth order 4 (60.0%) and children that have blood group B (51.3%), however, there is no significant difference in the distribution of between gender (χ2 = 0.634, df = 1, > 0.05). There was a point mutation in the codon position 557 where the amino acid Alanine was replaced by Serine in the The presence of mutant genes and point mutation in the gene of among children, calls for development of innovative drugs targeted on these resistant strains.
耐药物的疟原虫一直是有效治疗疟疾的主要障碍。因此，本研究旨在评估尼日利亚阿库雷州妇幼医院5岁及以下儿童中13号染色体上的抗药凯尔希蛋白基因（Kelch 13）和多药耐药（）突变基因的分布情况。通过静脉穿刺无菌采集500名儿童的血液，制备厚薄血涂片。使用结构化问卷从受访者那里获取人口统计学数据。从500个样本中随机选取200个疟疾阳性样本进行PCR分析，以检测和突变基因。结果显示，在500名受访者中，288名（57.6%）为男性，212名（42.4%）为女性。Pfmdr1分布情况如下：混合组（突变体/野生型）38.5%，突变基因35.5%，野生基因20.5%，5.5%的感染儿童中不存在耐药基因。基因的混合组在婴儿中比例较高（51.9%），在出生顺序为4的儿童中（60.0%）以及血型为B的儿童中（51.3%），然而，基因在性别之间的分布没有显著差异（χ2 = 0.634，自由度 = 1，P > 0.05）。在密码子位置557处存在点突变，其中丙氨酸被丝氨酸取代。儿童中存在突变基因和基因的点突变，这就需要开发针对这些耐药菌株的创新药物。
