从……分离出的LM1185的免疫刺激活性。
Immunostimulatory activity of LM1185 isolated from .
作者信息
Cho Yoonjeong, Han Hyeon Tak, Kim Tae-Rahk, Sohn Minn, Park Young-Seo
机构信息
Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.
Center for Research and Development, LACTOMASON, Jinju, 52840 Republic of Korea.
出版信息
Food Sci Biotechnol. 2022 Nov 13;32(4):497-506. doi: 10.1007/s10068-022-01199-5. eCollection 2023 Mar.
UNLABELLED
The lactic acid bacteria, subsp. LM1185 was isolated from . Strain LM1185 showed 50.5% of acid tolerance at pH 2.5 for 2 h and 30.4% of 0.3% (w/v) bile salt tolerance for 24 h. The antioxidant activity of this strain was measured at 99.4% of 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity. When RAW 264.7 macrophage cells were treated with strain LM1185, there was no observed cytotoxicity. This strain showed high nitric oxide production and mRNA expression levels of cytokines such as tumor necrosis factor-α and inducible nitric oxide synthase (iNOS). The nuclear factor-kB signaling pathway was activated by this strain resulting in the production of iNOS and cyclooxygenase-2 determined by western blotting. The present results indicated that subsp. LM1185 could be used as potential probiotics and may play a crucial role in the immunostimulatory effect on macrophages.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01199-5.
未标记
乳酸菌亚种LM1185是从……分离得到的。菌株LM1185在pH 2.5条件下2小时的耐酸性为50.5%，在0.3%（w/v）胆盐条件下24小时的耐胆盐性为30.4%。该菌株的抗氧化活性通过2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)自由基清除活性测定为99.4%。当用菌株LM1185处理RAW 264.7巨噬细胞时，未观察到细胞毒性。该菌株显示出高的一氧化氮产生以及细胞因子如肿瘤坏死因子-α和诱导型一氧化氮合酶（iNOS）的mRNA表达水平。通过蛋白质印迹法测定，该菌株激活了核因子-κB信号通路，导致iNOS和环氧化酶-2的产生。目前的结果表明，乳酸菌亚种LM1185可作为潜在的益生菌，可能在对巨噬细胞的免疫刺激作用中发挥关键作用。
补充信息
在线版本包含可在10.1007/s10068-022-01199-5获取的补充材料。
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