使用Airyscan共聚焦显微镜观察植物根部发育中的木质部导管新出现的次生加厚部位的纤维素合酶。
Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy.
作者信息
Wightman Raymond
机构信息
Microscopy Core Facility, Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.
出版信息
Cell Surf. 2023 Feb 28;9:100103. doi: 10.1016/j.tcsw.2023.100103. eCollection 2023 Dec.
Abstract
Movement of cellulose synthase particles have so far been observed on the plant epidermis that are amenable to confocal imaging, yielding appreciable signal and resolution to observe small plasma membrane-localised particles. Presented here is a method, using airyscan confocal microscopy, that permits similar information to be obtained at depth within the developing protoxylem vessels of intact roots.
摘要
迄今为止，已在适合共聚焦成像的植物表皮上观察到纤维素合酶颗粒的运动，从而产生可观的信号和分辨率，以观察小的质膜定位颗粒。本文介绍了一种使用Airyscan共聚焦显微镜的方法，该方法能够在完整根的发育中的原生木质部导管深处获得类似的信息。
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