Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, Japan.
Department of Pathology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
Front Immunol. 2023 Feb 23;14:1034978. doi: 10.3389/fimmu.2023.1034978. eCollection 2023.
Neutralizing antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being developed world over. We investigated the possibility of producing artificial antibodies from the formalin fixation and paraffin-embedding (FFPE) lung lobes of a patient who died by coronavirus disease 2019 (COVID-19). The B-cell receptors repertoire in the lung tissue where SARS-CoV-2 was detected were considered to have highly sensitive virus-neutralizing activity, and artificial antibodies were produced by combining the most frequently detected heavy and light chains. Some neutralizing effects against the SARS-CoV-2 were observed, and mixing two different artificial antibodies had a higher tendency to suppress the virus. The neutralizing effects were similar to the immunoglobulin G obtained from healthy donors who had received a COVID-19 mRNA vaccine. Therefore, the use of FFPE lung tissue, which preserves the condition of direct virus sensitization, to generate artificial antibodies may be useful against future unknown infectious diseases.
目前，全世界都在开发针对严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的中和抗体。我们研究了从死于 2019 冠状病毒病（COVID-19）的患者的福尔马林固定石蜡包埋（FFPE）肺组织中产生人工抗体的可能性。在检测到 SARS-CoV-2 的肺组织中，B 细胞受体库被认为具有高度敏感的病毒中和活性，并通过组合最频繁检测到的重链和轻链来产生人工抗体。观察到对 SARS-CoV-2 的一些中和作用，并且混合两种不同的人工抗体具有更高的抑制病毒的趋势。中和作用与从接受 COVID-19 mRNA 疫苗的健康供体获得的免疫球蛋白 G 相似。因此，使用保存直接病毒敏感状态的 FFPE 肺组织来产生人工抗体可能对未来未知的传染病有用。
