Scientific Research Institute, Yueyang Maternal-Child Medicine Health Hospital, Yueyang, China.
Hengyang Maternal and Child Health Hospital, Hengyang, China.
Mol Cell Biochem. 2023 Dec;478(12):2621-2627. doi: 10.1007/s11010-023-04688-1. Epub 2023 Mar 13.
It has been shown that the circular RNA (circRNA) circPTK2 modulates many types of diseases. However, the possible functions as well as the molecular mechanisms of circPTK2 in preeclampsia (PE) and their effects on trophoblast are unknown. Herein, we obtained the placental tissues from 20 pregnant women with PE who delivered in the Yueyang Maternal Child Medicine Health Hospital between 2019 and 2021 to serve as the PE group, and a normal group was composed of 20 healthy pregnant women with normal prenatal examinations. The circPTK2 level was significantly reduced in tissues from the PE group. The expression and localization of circPTK2 were verified using RT-qPCR. CircPTK2 silencing inhibited HTR-8/SVneo growth and migration in vitro. To investigate the underlying mechanism of circPTK2 in PE progression, dual-luciferase reporter assays were conducted. It was found that circPTK2 and WNT7B could bind directly to miR-619, and that circPTK2 affected WNT7B expression by sponging miR-619. To conclude, this study identified the functions and mechanisms of the circPTK2/miR-619/WNT7B axis in PE progression. In this way, circPTK2 has the potential to be used both in diagnostic and therapeutic settings for PE.
已经表明，环状 RNA (circRNA) circPTK2 调节多种疾病。然而，circPTK2 在子痫前期 (PE) 中的可能功能以及分子机制及其对滋养层的影响尚不清楚。在此，我们从 2019 年至 2021 年在岳阳妇幼保健院分娩的 20 名患有 PE 的孕妇中获得胎盘组织作为 PE 组，正常组由 20 名正常产前检查的健康孕妇组成。PE 组组织中的 circPTK2 水平明显降低。使用 RT-qPCR 验证了 circPTK2 的表达和定位。CircPTK2 沉默抑制了体外 HTR-8/SVneo 的生长和迁移。为了研究 circPTK2 在 PE 进展中的潜在机制，进行了双荧光素酶报告基因检测。结果发现，circPTK2 和 WNT7B 可以直接与 miR-619 结合，circPTK2 通过海绵吸附 miR-619 影响 WNT7B 的表达。总之，本研究鉴定了 circPTK2/miR-619/WNT7B 轴在 PE 进展中的功能和机制。这样，circPTK2 有可能在 PE 的诊断和治疗中得到应用。
