Ning Baile, Wang Zhifang, Wu Qian, Deng Qiyue, Yang Qing, Gao Jing, Fu Wen, Deng Ying, Wu Bingxin, Huang Xichang, Mei Jilin, Fu Wenbin
Guangzhou University of Chinese Medicine, Guangzhou, China; The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Guangzhou University of Chinese Medicine, Guangzhou, China.
Brain Res. 2023 Jun 1;1808:148320. doi: 10.1016/j.brainres.2023.148320. Epub 2023 Mar 11.
Acupuncture is a good treatment for depression in Parkinson's disease (DPD), so the possible mechanism of acupuncture in the treatment of DPD was explored in this study. Firstly, observing the behavioral changes of the DPD rat model, the regulation of monoamine neurotransmitters dopamine (DA) and 5-hydroxytryptamine (5-HT) in the midbrain, the change of α-synuclein (α-syn) in the striatum, the efficacy of acupuncture in the treatment of DPD was discussed. Secondly, autophagy inhibitors and activators were selected to judge the effect of acupuncture on autophagy in the DPD rat model. Finally, an mTOR inhibitor was used to observe the effect of acupuncture on the mTOR pathway in the DPD rat model. The results showed that acupuncture could improve the motor and depressive symptoms of DPD model rats, increase the content of DA and 5-HT, and decrease the content of ɑ-syn in the striatum. Acupuncture inhibited the expression of autophagy in the striatum of DPD model rats. At the same time, acupuncture upregulates p-mTOR expression, inhibits autophagy, and promotes synaptic protein expression. Therefore, we concluded that acupuncture might improve the behavior of DPD model rats by activating the mTOR pathway, inhibiting autophagy from removing α-syn and repairing synapses.
针刺是帕金森病伴发抑郁（DPD）的一种有效治疗方法，因此本研究探讨了针刺治疗DPD的可能机制。首先，通过观察DPD大鼠模型的行为变化、中脑单胺类神经递质多巴胺（DA）和5-羟色胺（5-HT）的调节情况、纹状体中α-突触核蛋白（α-syn）的变化，探讨针刺治疗DPD的疗效。其次，选用自噬抑制剂和激活剂来判断针刺对DPD大鼠模型自噬的影响。最后，使用mTOR抑制剂观察针刺对DPD大鼠模型mTOR通路的影响。结果显示，针刺可改善DPD模型大鼠的运动和抑郁症状，增加DA和5-HT的含量，并降低纹状体中ɑ-syn的含量。针刺抑制了DPD模型大鼠纹状体中自噬的表达。同时，针刺上调p-mTOR表达，抑制自噬，并促进突触蛋白表达。因此，我们得出结论，针刺可能通过激活mTOR通路、抑制自噬清除α-syn以及修复突触来改善DPD模型大鼠的行为。
