将生物医学肌肉组织工程再用于细胞农业：挑战与机遇。
Repurposing biomedical muscle tissue engineering for cellular agriculture: challenges and opportunities.
机构信息
Department of Biomedical Engineering, University of Connecticut, Farmington, CT, USA.
Department of Biomedical Engineering, University of Connecticut, Farmington, CT, USA.
出版信息
Trends Biotechnol. 2023 Jul;41(7):887-906. doi: 10.1016/j.tibtech.2023.02.002. Epub 2023 Mar 11.
Abstract
Cellular agriculture is an emerging field rooted in engineering meat-mimicking cell-laden structures using tissue engineering practices that have been developed for biomedical applications, including regenerative medicine. Research and industrial efforts are focused on reducing the cost and improving the throughput of cultivated meat (CM) production using these conventional practices. Due to key differences in the goals of muscle tissue engineering for biomedical versus food applications, conventional strategies may not be economically and technologically viable or socially acceptable. In this review, these two fields are critically compared, and the limitations of biomedical tissue engineering practices in achieving the important requirements of food production are discussed. Additionally, the possible solutions and the most promising biomanufacturing strategies for cellular agriculture are highlighted.
摘要
细胞农业是一个新兴领域，其基础是使用组织工程实践来构建模拟肉类的细胞结构，这些实践已经为生物医学应用（包括再生医学）所开发。研究和工业界的重点是通过这些传统实践来降低培养肉（CM）生产的成本并提高产量。由于生物医学和食品应用的肌肉组织工程目标存在关键差异，因此传统策略在经济和技术上可能不可行或在社会上不可接受。在这篇综述中，对这两个领域进行了批判性比较，并讨论了生物医学组织工程实践在实现食品生产的重要要求方面的局限性。此外，还强调了细胞农业的可能解决方案和最有前途的生物制造策略。
相似文献
1
Repurposing biomedical muscle tissue engineering for cellular agriculture: challenges and opportunities.将生物医学肌肉组织工程再用于细胞农业：挑战与机遇。
Trends Biotechnol. 2023 Jul;41(7):887-906. doi: 10.1016/j.tibtech.2023.02.002. Epub 2023 Mar 11.
2
Bioengineering Outlook on Cultivated Meat Production.实验室培育肉生产的生物工程展望
Micromachines (Basel). 2022 Feb 28;13(3):402. doi: 10.3390/mi13030402.
3
Cell Sources for Cultivated Meat: Applications and Considerations throughout the Production Workflow.培养肉的细胞来源：在整个生产工作流程中的应用和考虑因素。
Int J Mol Sci. 2021 Jul 13;22(14):7513. doi: 10.3390/ijms22147513.
4
Advancing our understanding of bioreactors for industrial-sized cell culture: health care and cellular agriculture implications.推进工业规模细胞培养生物反应器的理解：医疗保健和细胞农业的影响。
Am J Physiol Cell Physiol. 2023 Sep 1;325(3):C580-C591. doi: 10.1152/ajpcell.00408.2022. Epub 2023 Jul 24.
5
Regenerative pharmacology: the future is now.再生药理学：未来已来。
Mol Interv. 2007 Apr;7(2):79-86. doi: 10.1124/mi.7.2.8.
6
Prospects and challenges for cell-cultured fat as a novel food ingredient.细胞培养脂肪作为一种新型食品成分的前景与挑战。
Trends Food Sci Technol. 2020 Apr;98:53-67. doi: 10.1016/j.tifs.2020.02.005. Epub 2020 Feb 11.
7
Tooth regeneration: challenges and opportunities for biomedical material research.牙齿再生：生物医学材料研究面临的挑战与机遇
Biomed Mater. 2006 Mar;1(1):R10-7. doi: 10.1088/1748-6041/1/1/R02. Epub 2006 Mar 1.
8
A scoping review of cultivated meat techno-economic analyses to inform future research directions for scaled-up manufacturing.培养肉技术经济分析的范围综述，为规模化生产的未来研究方向提供信息。
Nat Food. 2024 Nov;5(11):901-910. doi: 10.1038/s43016-024-01061-3. Epub 2024 Oct 18.
9
Integrating biomaterials and food biopolymers for cultured meat production.整合生物材料与食品生物聚合物用于人造肉生产。
Acta Biomater. 2021 Apr 1;124:108-129. doi: 10.1016/j.actbio.2021.01.017. Epub 2021 Jan 17.
10
Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.用于 3D 培养肉的支架生物材料：前景与挑战。
Adv Sci (Weinh). 2022 Jan;9(3):e2102908. doi: 10.1002/advs.202102908. Epub 2021 Nov 16.
引用本文的文献
1
Scaffold Biomaterials in the Development of Cultured Meat: A Review.培养肉开发中的支架生物材料：综述
Food Sci Anim Resour. 2025 May;45(3):688-710. doi: 10.5851/kosfa.2025.e13. Epub 2025 May 1.
2
Biodegradable Oxygen-Generating Microneedle Patches for Regenerative Medicine Applications.用于再生医学应用的可生物降解产氧微针贴片
Adv Nanobiomed Res. 2025 Jan;5(1):2400093. doi: 10.1002/anbr.202400093. Epub 2024 Nov 27.
3
Establishment and characterization of hTERT-immortalized porcine muscle stem cells, and their prospective uses.hTERT永生化猪肌肉干细胞的建立、鉴定及其潜在用途。
Food Sci Biotechnol. 2024 Dec 11;34(7):1597-1604. doi: 10.1007/s10068-024-01785-9. eCollection 2025 Apr.
4
A Review on the Application of Animal-Based Materials Using Three-Dimensional (3D) Printing and Protein Restructuring Technologies.基于动物材料的三维（3D）打印与蛋白质重组技术应用综述
Food Sci Anim Resour. 2025 Jan;45(1):282-302. doi: 10.5851/kosfa.2024.e132. Epub 2025 Jan 1.
5
A robotic arm with open-source reconstructive workflow for bioprinting of patient-specific scaffolds.一种具有开源重建工作流程的机械臂，用于生物打印患者特异性支架。
Appl Phys Rev. 2024 Dec;11(4):041402. doi: 10.1063/5.0197123.
6
Laminin as a Key Extracellular Matrix for Proliferation, Differentiation, and Maturation of Porcine Muscle Stem Cell Cultivation.层粘连蛋白作为猪肌肉干细胞培养增殖、分化和成熟的关键细胞外基质。
Food Sci Anim Resour. 2024 May;44(3):710-722. doi: 10.5851/kosfa.2024.e27. Epub 2024 May 1.
7
Cultivation of Bovine Mesenchymal Stem Cells on Plant-Based Scaffolds in a Macrofluidic Single-Use Bioreactor for Cultured Meat.在用于培养肉的一次性宏观流体生物反应器中，在植物基支架上培养牛间充质干细胞。
Foods. 2024 Apr 28;13(9):1361. doi: 10.3390/foods13091361.
8
Biofabrication's Contribution to the Evolution of Cultured Meat.生物制造对培养肉发展的贡献。
Adv Healthc Mater. 2024 May;13(13):e2304058. doi: 10.1002/adhm.202304058. Epub 2024 Feb 17.
本文引用的文献
1
A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation.一种用于培养肉生产的无血清培养基配方可在无血清饥饿的情况下支持牛卫星细胞分化。
Nat Food. 2022 Jan;3(1):74-85. doi: 10.1038/s43016-021-00419-1. Epub 2022 Jan 13.
2
3D-Printed Prolamin Scaffolds for Cell-Based Meat Culture.用于基于细胞的肉类培养的3D打印醇溶蛋白支架
Adv Mater. 2023 Jan;35(2):e2207397. doi: 10.1002/adma.202207397. Epub 2022 Nov 27.
3
Efficient Myogenic/Adipogenic Transdifferentiation of Bovine Fibroblasts in a 3D Bioprinting System for Steak-Type Cultured Meat Production.用于牛排型培养肉生产的 3D 生物打印系统中牛成纤维细胞的高效成肌/成脂转分化。
Adv Sci (Weinh). 2022 Nov;9(31):e2202877. doi: 10.1002/advs.202202877. Epub 2022 Oct 3.
4
Tailoring a Gelatin/Agar Matrix for the Synergistic Effect with Cells to Produce High-Quality Cultured Meat.定制一种明胶/琼脂基质，以与细胞产生协同作用，生产高质量的培养肉。
ACS Appl Mater Interfaces. 2022 Aug 24;14(33):38235-38245. doi: 10.1021/acsami.2c10988. Epub 2022 Aug 14.
5
Production of cultured meat by culturing porcine smooth muscle cells in vitro with food grade peanut wire-drawing protein scaffold.通过在体外使用食品级花生拉丝蛋白支架培养猪平滑肌细胞来生产 cultured meat（此处cultured meat可能是“培养肉”，但该词较新，具体准确含义可能需结合更多背景信息）
Food Res Int. 2022 Sep;159:111561. doi: 10.1016/j.foodres.2022.111561. Epub 2022 Jun 23.
6
Production of cultured meat from pig muscle stem cells.利用猪肌肉干细胞生产培养肉。
Biomaterials. 2022 Aug;287:121650. doi: 10.1016/j.biomaterials.2022.121650. Epub 2022 Jul 18.
7
Emulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat.乳液模板化微球具有可调的刚度和拓扑结构：作为培养肉用可食用微载体的应用。
Biomaterials. 2022 Aug;287:121669. doi: 10.1016/j.biomaterials.2022.121669. Epub 2022 Jul 12.
8
Bioengineered Lab-Grown Meat-like Constructs through 3D Bioprinting of Antioxidative Protein Hydrolysates.通过抗氧化蛋白水解物的 3D 生物打印技术生产类生物工程培养肉样构建体。
ACS Appl Mater Interfaces. 2022 Aug 3;14(30):34513-34526. doi: 10.1021/acsami.2c10620. Epub 2022 Jul 18.
9
Edible films for cultivated meat production.用于培养肉生产的可食用薄膜。
Biomaterials. 2022 Aug;287:121659. doi: 10.1016/j.biomaterials.2022.121659. Epub 2022 Jul 6.
10
Harvest of quality-controlled bovine myogenic cells and biomimetic bovine muscle tissue engineering for sustainable meat production.用于可持续肉类生产的优质控制牛成肌细胞的收获及仿生牛肌肉组织工程
Biomaterials. 2022 Aug;287:121649. doi: 10.1016/j.biomaterials.2022.121649. Epub 2022 Jun 25.
Zotero 插件