Chen Xiaohong, Xiang Fangfang, Cao Xuesen, Zou Jianzhou, Zhang Boheng, Ding Xiaoqiang
Shanghai Institute of Kidney and Dialysis, Shanghai, China.
Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China.
Transl Cancer Res. 2023 Feb 28;12(2):367-374. doi: 10.21037/tcr-22-2042. Epub 2023 Jan 5.
Though p-cresol exists at a low concentration in the blood, it accumulates in various organs of uremic patients. Previous research has shown that the p-cresol promoted bladder cancer cell invasion and migration. This study aims to see if p-cresol had similar effects on kidney cancer cells and liver cancer cells.
For 48 hours, 786-O human renal cancer cells and HepG2 human liver cancer cells were treated with p-cresol at concentrations of 0, 10, 20, 40, and 70 µM. The effects of p-cresol on cell viability, apoptosis, migration, and invasion were then analyzed using the CCK-8, TUNEL, and Transwell migration/invasion assays, respectively.
P-cresol at 0 to 70 µM for 48 hours had no significant toxic effects on 786-O cells or HepG2 cells. We chose 40 µM p-cresol for 48 hours for the following experiment. The viability and proliferation of 786-O cells and HepG2 cells were unaffected after 48 hours of treatment, with 40 µM p-cresol. However, 40 µM p-cresol for 48 hours promoted HepG2 cell migration and invasion but did not have the same effect on the 786-O cell line.
P-cresol may be responsible for HepG2 cells' malignant biological behavior. Because the liver is the primary site of p-cresol metabolism, it is important to study the responses of cancer cells in the liver to p-cresol.
尽管对甲酚在血液中的浓度较低，但它会在尿毒症患者的各个器官中蓄积。先前的研究表明，对甲酚可促进膀胱癌细胞的侵袭和迁移。本研究旨在观察对甲酚对肾癌细胞和肝癌细胞是否有类似作用。
将786 - O人肾癌细胞和HepG2人肝癌细胞分别用浓度为0、10、20、40和70 μM的对甲酚处理48小时。然后分别使用CCK - 8、TUNEL和Transwell迁移/侵袭试验分析对甲酚对细胞活力、凋亡、迁移和侵袭的影响。
0至70 μM的对甲酚处理48小时对786 - O细胞或HepG2细胞无明显毒性作用。我们选择40 μM对甲酚处理48小时进行后续实验。用40 μM对甲酚处理48小时后，786 - O细胞和HepG2细胞的活力和增殖未受影响。然而，40 μM对甲酚处理48小时可促进HepG2细胞的迁移和侵袭，但对786 - O细胞系无此作用。
对甲酚可能与HepG2细胞的恶性生物学行为有关。由于肝脏是对甲酚代谢的主要部位，研究肝脏中的癌细胞对对甲酚的反应很重要。
