Imanishi Daiki, Takahashi Shouji
Department of Material Science and Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Data Brief. 2023 Feb 23;47:109008. doi: 10.1016/j.dib.2023.109008. eCollection 2023 Apr.
The yeast (previously ) strain UJ1 produces d-aspartate oxidase (DDO) only in the presence of d-aspartate in culture media. This article provides RNA-sequencing data to identify the differentially expressed genes (DEGs) in the yeast cells grown between l- and d-aspartate. RNA samples were prepared from the yeast cells grown in a culture medium containing 30 mM d-aspartate or l-aspartate as the sole carbon source and subjected to RNA sequencing on Illumina NovaSeq6000 platform. The clean reads obtained by removing adaptor sequences and low-quality reads from raw reads were submitted to the Sequence Read Archive (SRA) database of the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJDB13570. The clean reads were subjected to differential gene expression analysis using DEGSeq to provide data on the upregulated and downregulated DEGs in the cells grown on d-aspartate. The DEGs were subjected to gene ontology (GO) and KEGG pathway enrichment analyses using GOSeq and KOBAS, respectively, to provide data on the possible biological functions of the DEGs. The data set obtained in this project might be helpful for further investigation of the effects of d-aspartate on cellular processes in yeast cells and other eukaryotic organisms.
酵母（先前的）菌株UJ1仅在培养基中存在d-天冬氨酸时才产生d-天冬氨酸氧化酶（DDO）。本文提供RNA测序数据，以鉴定在l-天冬氨酸和d-天冬氨酸中生长的酵母细胞中的差异表达基因（DEG）。RNA样本是从在含有30 mM d-天冬氨酸或l-天冬氨酸作为唯一碳源的培养基中生长的酵母细胞中制备的，并在Illumina NovaSeq6000平台上进行RNA测序。通过从原始读数中去除接头序列和低质量读数而获得的干净读数已提交至美国国立生物技术信息中心（NCBI）的序列读数存档（SRA）数据库，生物项目登录号为PRJDB13570。使用DEGSeq对干净读数进行差异基因表达分析，以提供在d-天冬氨酸上生长的细胞中上调和下调的DEG的数据。分别使用GOSeq和KOBAS对DEG进行基因本体（GO）和KEGG途径富集分析，以提供有关DEG可能的生物学功能的数据。该项目中获得的数据集可能有助于进一步研究d-天冬氨酸对酵母细胞和其他真核生物细胞过程的影响。
