Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
Int J Mol Sci. 2021 Jan 3;22(1):425. doi: 10.3390/ijms22010425.
Tissue engineering has attracted significant attention since the 1980s, and the applications of tissue engineering have been expanding. To produce a cell-dense tissue, cell sheet technology has been studied as a promising strategy. Fundamental techniques involving tissue engineering are mainly introduced in this review. First, the technologies to fabricate a cell sheet were reviewed. Although temperature-responsive polymer-based technique was a trigger to establish and spread cell sheet technology, other methodologies for cell sheet fabrication have also been reported. Second, the methods to improve the function of the cell sheet were investigated. Adding electrical and mechanical stimulation on muscle-type cells, building 3D structures, and co-culturing with other cell species can be possible strategies for imitating the physiological situation under in vitro conditions, resulting in improved functions. Finally, culture methods to promote vasculogenesis in the layered cell sheets were introduced with in vivo, ex vivo, and in vitro bioreactors. We believe the present review that shows and compares the fundamental technologies and recent advances for cell-sheet-based tissue engineering should promote further development of tissue engineering. The development of cell sheet technology should promote many bioengineering applications.
组织工程自 20 世纪 80 年代以来引起了广泛关注，其应用范围不断扩大。为了生成细胞密集的组织，细胞片层技术已被作为一种很有前途的策略进行研究。本综述主要介绍了涉及组织工程的基本技术。首先，回顾了制备细胞片层的技术。尽管基于温度响应性聚合物的技术是建立和推广细胞片层技术的触发因素，但也有其他用于制备细胞片层的方法。其次，研究了提高细胞片层功能的方法。对肌型细胞施加电和机械刺激、构建 3D 结构以及与其他细胞物种共培养是模拟体外生理条件下提高功能的可行策略。最后，介绍了促进层状细胞片中血管生成的培养方法，包括体内、体外和体外生物反应器。我们相信，本综述展示和比较了基于细胞片层的组织工程的基本技术和最新进展，将促进组织工程的进一步发展。细胞片层技术的发展将推动许多生物工程应用。
