菌株 S1-10 释放的挥发性有机化合物对具有熏杀活性。

Volatile Organic Compounds of Strain S1-10 Exhibit Fumigant Activity Against .

机构信息

Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, China.

Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University, Shandong 257347, China.

出版信息

Plant Dis. 2023 Oct;107(10):3057-3063. doi: 10.1094/PDIS-10-22-2391-RE. Epub 2023 Oct 12.

DOI:10.1094/PDIS-10-22-2391-RE

PMID:36916837

Abstract

Root-knot nematodes (RKNs) are highly specialized parasites that cause significant yield losses worldwide. In this study, we isolated strain S1-10 from the rhizosphere soil of Rosc. plants and evaluated its fumigant activity against . S1-10 exhibited a strong repellent effect on second-stage juveniles (J2s) of , and in vitro assays indicated that S1-10 volatile organic compounds (VOCs) suppressed J2 activity and egg hatching. Under greenhouse conditions, 71 and 79% reductions of nematodes and eggs were detected on plants treated with S-10 VOCs compared with controls. Ten VOCs were identified through gas chromatography and mass spectrometry (GC-MS), of which 2-(methylamino)-ethanol (2-ME) had strong fumigant activity against J2s of , with an LC value of 1.5 mM at 12 h. These results indicate that S1-10 represents a potential novel biocontrol agent for RKNs.

摘要

根结线虫（RKNs）是高度专业化的寄生虫，在全球范围内造成了重大的产量损失。在这项研究中，我们从 Rosc. 植物的根际土壤中分离出了 S1-10 菌株，并评估了其对的熏蒸活性。S1-10 对的第二代幼虫（J2s）表现出强烈的驱避作用，体外试验表明 S1-10 挥发性有机化合物（VOCs）抑制了 J2 的活性和卵的孵化。在温室条件下，与对照相比，用 S-10VOCs 处理的植物上检测到线虫和卵的减少分别为 71%和 79%。通过气相色谱和质谱联用（GC-MS）鉴定出了 10 种 VOCs，其中 2-（甲氨基）-乙醇（2-ME）对的 J2s 具有强烈的熏蒸活性，在 12 小时时 LC 值为 1.5mM。这些结果表明 S1-10 代表了一种防治 RKNs 的潜在新型生物防治剂。