State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.
Molecules. 2021 Dec 24;27(1):103. doi: 10.3390/molecules27010103.
Plant-parasitic nematodes cause severe losses to crop production and economies all over the world. MCCC 1K02966, a deep-sea bacterium, was obtained from the Southwest Indian Ocean and showed nematicidal and fumigant activities against in vitro. The nematicidal volatile organic compounds (VOCs) from the fermentation broth of MCCC 1K02966 were investigated further using solid-phase microextraction gas chromatography-mass spectrometry. Four VOCs, namely, pentane, 1-butanol, methyl thioacetate, and dimethyl disulfide, were identified in the fermentation broth. Among these VOCs, methyl thioacetate exhibited multiple nematicidal activities, including contact nematicidal, fumigant, and repellent activities against . Methyl thioacetate showed a significant contact nematicidal activity with 87.90% mortality at 0.01 mg/mL by 72 h, fumigant activity in mortality 91.10% at 1 mg/mL by 48 h, and repellent activity at 0.01-10 mg/mL. In addition, methyl thioacetate exhibited 80-100% egg-hatching inhibition on the 7th day over the range of 0.5 mg/mL to 5 mg/mL. These results showed that methyl thioacetate from MCCC 1K02966 control with multiple nematicidal modes and can be used as a potential biological control agent.
植物寄生线虫对全世界的作物生产和经济造成严重损失。从西南印度洋获得的深海细菌 MCCC 1K02966 对 具有杀线虫和熏蒸活性。使用固相微萃取气相色谱-质谱法进一步研究了 MCCC 1K02966 发酵液中的杀线虫挥发性有机化合物 (VOCs)。在发酵液中鉴定出四种 VOCs，分别为戊烷、1-丁醇、甲基硫代乙酸酯和二甲基二硫。在这些 VOCs 中，甲基硫代乙酸酯对 表现出多种杀线虫活性，包括接触杀线虫、熏蒸和驱避活性。甲基硫代乙酸酯在 72 小时内以 0.01 mg/mL 的浓度表现出显著的接触杀线虫活性，死亡率为 87.90%，在 48 小时内以 1 mg/mL 的浓度表现出熏蒸活性，死亡率为 91.10%，在 0.01-10 mg/mL 的浓度下表现出驱避活性。此外，甲基硫代乙酸酯在 0.5-5 mg/mL 范围内对 7 天龄的卵孵化具有 80-100%的抑制作用。这些结果表明，MCCC 1K02966 产生的甲基硫代乙酸酯通过多种杀线虫模式控制 ，可作为一种有潜力的生物防治剂。
