Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh.
Department of Pathology, Division of Clinical Chemistry, Toxicology and Therapeutic Drug Monitoring Laboratory, University of Pittsburgh Medical Center Presbyterian/Shadyside.
Ther Drug Monit. 2023 Aug 1;45(4):431-462. doi: 10.1097/FTD.0000000000001083. Epub 2023 Mar 6.
A novel microsampling device called Volumetric Absorptive microsampling (VAMS), developed in 2014, appears to have resolved the sample inhomogeneity inherent to dried blood spots, with improved precision in the volume of sample collected for measuring drug concentration. A literature search was conducted to identify several analytical and pharmacokinetic studies that have used VAMS in recent years.
The key factors for proper experimental design and optimization of the extraction of drugs and metabolites of interest from the device were summarized. This review focuses on VAMS and elaborates on bioanalytical factors, method validation steps, and scope of this technique in clinical practice.
The promising microsampling method VAMS is especially suited for conducting pharmacokinetic studies with very small volumes of blood, especially in special patient populations. Clinical validation of every VAMS assay must be conducted prior to the routine practical implementation of this method.
一种名为容积吸附微采样（VAMS）的新型微采样装置，于 2014 年开发，似乎解决了干血斑固有的样本不均匀性问题，提高了测量药物浓度时采集样本量的精密度。进行了文献检索，以确定近年来使用 VAMS 的几项分析和药代动力学研究。
总结了从装置中适当设计和优化提取药物和感兴趣代谢物的实验的关键因素。本综述重点介绍了 VAMS，并详细阐述了生物分析因素、方法验证步骤以及该技术在临床实践中的应用范围。
有前途的微采样方法 VAMS 特别适合进行小体积血液的药代动力学研究，尤其是在特殊患者人群中。在常规实际实施该方法之前，必须对每个 VAMS 检测进行临床验证。
