Pagan Erik, Stefanek Evan, Seyfoori Amir, Razzaghi Mahmood, Chehri Behnad, Mousavi Ali, Arnaldi Pietro, Ajji Zineb, Dartora Daniela Ravizzoni, Dabiri Seyed Mohammad Hossein, Nuyt Anne Monique, Khademhosseini Ali, Savoji Houman, Akbari Mohsen
Laboratory for Innovations in Microengineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria BC V8P 5C2, Canada.
Institute of Biomedical Engineering, Department of Pharmacology and Physiology, Faculty of Medicine, University of Montreal, Montreal QC H3T 1J4, Canada.
Biofabrication. 2023 May 2;15(3). doi: 10.1088/1758-5090/acc42c.
bioprinting-the process of depositing bioinks at a defected area, has recently emerged as a versatile technology for tissue repair and restorationsite-specific delivery of pro-healing constructs. The ability to print multiple materialsis an exciting approach that allows simultaneous or sequential dispensing of different materials and cells to achieve tissue biomimicry. Herein, we report a modular handheld bioprinter that deposits a variety of bioinkswith exquisite control over their physical and chemical properties. Combined stereolithography 3D printing and microfluidic technologies allowed us to develop a novel low-priced handheld bioprinter. The ergonomic design of the handheld bioprinter facilitate the shape-controlled biofabrication of multi-component fibers with different cross-sectional shapes and material compositions. Furthermore, the capabilities of the produced fibers in the local delivery of therapeutic agents was demonstrated by incorporating drug-loaded microcarriers, extending the application of the printed fibers to on-demand, temporal, and dosage-control drug delivery platforms. Also, the versatility of this platform to produce biosensors and wearable electronics was demonstrated via incorporating conductive materials and integrating pH-responsive dyes. The handheld printer's efficacy in generating cell-laden fibers with high cell viability for site-specific cell delivery was shown by producing single-component and multi-component cell-laden fibers. In particular, the multi-component fibers were able to model the invasion of cancer cells into the adjacent tissue.
生物打印——即在缺损区域沉积生物墨水的过程，最近已成为一种用于组织修复和恢复的通用技术，能够实现促愈合构建体的位点特异性递送。打印多种材料的能力是一种令人兴奋的方法，它允许同时或顺序地分配不同的材料和细胞，以实现组织仿生。在此，我们报告了一种模块化手持式生物打印机，它能够精确控制各种生物墨水的物理和化学性质并进行沉积。结合立体光刻3D打印和微流控技术，使我们能够开发出一种新型的低价手持式生物打印机。手持式生物打印机的人体工程学设计有助于对具有不同横截面形状和材料成分的多组分纤维进行形状控制的生物制造。此外，通过掺入载药微载体，证明了所生产的纤维在局部递送治疗剂方面的能力，从而将打印纤维的应用扩展到按需、定时和剂量控制的药物递送平台。而且，通过掺入导电材料和整合pH响应染料，展示了该平台生产生物传感器和可穿戴电子产品的多功能性。通过生产单组分和多组分载细胞纤维，证明了手持式打印机在生成具有高细胞活力的载细胞纤维用于位点特异性细胞递送方面的功效。特别是，多组分纤维能够模拟癌细胞向邻近组织的侵袭。
