Alsafi Alaa, AlKaabi Siham Jasim
Department of Biology, Faculty of Education for Girls, Kufa University, Kufa, Iraq.
Cell Biochem Funct. 2023 Apr;41(3):365-374. doi: 10.1002/cbf.3788. Epub 2023 Mar 14.
The current study aimed to investigate the inhibitory activity of aqueous extracts of different plant parts of Rosa damascena, represented by the whole rose, petals, and calyces, against clinical isolates of Staphylococcus spp., Escherichia coli, and Klebsiella pneumoniae, and the inhibition of the bacterial cell. The isolates were obtained from the advanced microbiology laboratory for postgraduate studies in the Department of Biology, Faculty of Education for Girls. They were isolated from urinary tract infections, which were subsequently subjected to diagnosis by the Vitek-2 compact system to confirm the type of bacteria as well as their sensitivity to antibiotics. The results obtained included Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus lentus, Staphylococcus saprophyticus, E. coli, and K. pneumoniae. A test was conducted to investigate the microbiological inhibitory activity of aqueous plant extracts of the whole rose, petals, and calyces using the well diffusion method and three concentrations of each aqueous extract (25, 50, and 100 mg/ml). The results showed the inhibitory ability of all concentrations of the different extracts toward Staphylococcus spp., and E. coli and K. pneumoniae bacteria were not affected by the different concentrations of the plant extract. The concentration of (100 mg/ml) for the aqueous extract was the most efficient in inhibiting growth compared to the other concentrations. The synergistic effect of three antibiotics was examined (Amoxicillin-clavulanate 10/20 μg, Piperacillin 100 μg, Trimethoprim-sulfamethoxazole 23.75/1.25 μg) and for all concentrations of the aqueous plant extract was investigated in both E. coli and K. pneumoniae, as it found a synergistic action between some of the antibiotics and extracts towards inhibiting the growth of the two bacterial isolates Resistance to the plant extract alone. Bacterial isolates showed a significant decrease in the rate of adhesion to epithelial cells isolated from urine samples of healthy women in the presence of the aqueous extract of whole rose, petals, and calyces at their three concentrations compared with the control treatment.
本研究旨在调查大马士革蔷薇不同植物部位（以整株玫瑰、花瓣和花萼为代表）的水提取物对金黄色葡萄球菌、大肠杆菌和肺炎克雷伯菌临床分离株的抑制活性以及对细菌细胞的抑制作用。这些分离株取自女子教育学院生物学系研究生高级微生物学实验室。它们从尿路感染中分离得到，随后通过Vitek - 2紧凑型系统进行诊断，以确认细菌类型及其对抗生素的敏感性。获得的结果包括金黄色葡萄球菌、溶血葡萄球菌、缓慢葡萄球菌、腐生葡萄球菌、大肠杆菌和肺炎克雷伯菌。采用打孔扩散法，使用每种水提取物的三种浓度（25、50和100毫克/毫升）对整株玫瑰、花瓣和花萼的水植物提取物的微生物抑制活性进行了测试。结果表明，不同提取物的所有浓度对葡萄球菌属均有抑制能力，而大肠杆菌和肺炎克雷伯菌不受植物提取物不同浓度的影响。与其他浓度相比，水提取物浓度为100毫克/毫升时对生长的抑制效果最为显著。检测了三种抗生素（阿莫西林 - 克拉维酸10/20微克、哌拉西林100微克、甲氧苄啶 - 磺胺甲恶唑23.75/1.25微克）的协同作用，并研究了所有浓度的水植物提取物对大肠杆菌和肺炎克雷伯菌的作用，结果发现某些抗生素与提取物之间存在协同作用，可抑制这两种细菌分离株的生长，单独对植物提取物产生抗性。与对照处理相比，在三种浓度的整株玫瑰、花瓣和花萼水提取物存在的情况下，细菌分离株对从健康女性尿液样本中分离的上皮细胞的粘附率显著降低。
