Kitagawa Daisuke, Kitano Taito, Furumori Madoka, Suzuki Soma, Shintani Yui, Suzuki Yuki, Nakano Akiyo, Nakano Ryuichi, Nishiyama Atsuko, Yoshida Sayaka, Yano Hisakazu, Maeda Koichi, Nakamura Fumihiko
Department of Laboratory Medicine, Nara Prefecture General Medical Center, Japan.
Department of Microbiology and Infectious Diseases, Nara Medical University, Japan.
Heliyon. 2023 Mar 11;9(3):e14424. doi: 10.1016/j.heliyon.2023.e14424. eCollection 2023 Mar.
We aimed to investigate the epidemiology of respiratory infections by season and age during the COVID-19 pandemic in a Japanese acute care hospital using multiplex PCR testing.
We detected 21 pathogens in specimens from outpatients with respiratory symptoms at the Nara Prefecture General Medical Center using the multiplex PCR-based FilmArray Respiratory Panel 2.1 (bioMérieux).
Of the 3177 cases, 1215 (38.2%) were infected with at least one causative virus, and 1641 viruses were detected. The most common viruses detected were human rhinovirus/enterovirus (n = 655) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (n = 264). Additionally, 321 (10.1%) of these cases were infected with two or more overlapping viruses. There were 23 cases of co-infection with SARS-CoV-2 and other viruses. In the winter months from December 2020 to March 2021, the number of detected viruses was relatively low, followed by the surge of human rhinovirus/enterovirus, respiratory syncytial virus (RSV), and parainfluenza type 3 in the spring and summer of 2021. While the number of human rhinovirus/entero-virus remained relatively high after the 2021 summer, the number of other viruses detected since September 2021 was low. After December 2021, the number of SARS-CoV-2 increased rapidly.
Continuous monitoring of the epidemiology of respiratory infection is important to understand the prolonged impact of the COVID-19 pandemic.
我们旨在通过多重聚合酶链反应检测，调查日本一家急症医院在新冠疫情期间呼吸道感染按季节和年龄划分的流行病学情况。
我们使用基于多重聚合酶链反应的FilmArray呼吸道病原体2.1检测板（生物梅里埃公司），对奈良县综合医疗中心有呼吸道症状的门诊患者标本中的21种病原体进行了检测。
在3177例病例中，1215例（38.2%）感染了至少一种致病病毒，共检测到1641种病毒。检测到的最常见病毒是人类鼻病毒/肠道病毒（n = 655）和严重急性呼吸综合征冠状病毒2（SARS-CoV-2）（n = 264）。此外，这些病例中有321例（10.1%）感染了两种或更多重叠病毒。有23例SARS-CoV-2与其他病毒的合并感染。在2020年12月至2021年3月的冬季，检测到的病毒数量相对较低，随后在2021年春夏，人类鼻病毒/肠道病毒、呼吸道合胞病毒（RSV）和3型副流感病毒激增。虽然2021年夏季过后人类鼻病毒/肠道病毒数量仍相对较高，但自2021年9月以来检测到的其他病毒数量较低。2021年12月之后，SARS-CoV-2的数量迅速增加。
持续监测呼吸道感染的流行病学情况对于了解新冠疫情的长期影响很重要。
