Animal Reproduction Division, ICAR- Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, 243122, India.
Assistant Professor, Dept. of ARGO, CV &AS, (MAFSU), Prabhani, 431 402, Maharashtra, India.
Zygote. 2023 Jun;31(3):246-252. doi: 10.1017/S0967199423000084. Epub 2023 Mar 15.
This study is a comparative analysis of the biochemical, hormonal, and mineral compositions of follicular fluid in preovulatory and cystic follicles of water buffalo (). In total, reproductive tracts from 215 buffalo along with intact ovaries were collected randomly from an abattoir. The incidence of cystic conditions found in this study was 3.72% (8/215), involving the right ovary in 62.5% of instances and the left ovary in 37.5% of instances during the non-breeding season. Follicular fluid was aspirated from preovulatory follicles (12-15 mm diameter, oestrogen-active, follicular phase or stage IV corpus luteum on one of the two ovaries, = 10) and cystic follicles (at least 20 mm diameter, no corpus luteum on any one of the two ovaries, = 8). The follicular fluid samples were assayed for biochemical components (uric acid, creatinine, blood urea nitrogen, cholesterol, total protein, glucose, ascorbic acid, and alkaline phosphatase), hormones (progesterone, estradiol, and insulin), and minerals (calcium, magnesium, phosphorus, copper, zinc, and cobalt). Cystic follicles had greater ( < 0.05) concentrations of creatinine, blood urea nitrogen, cholesterol, progesterone, copper, zinc, and cobalt, and lesser ( < 0.05) concentrations of uric acid, glucose, ascorbic acid, estradiol, insulin, calcium, magnesium, and phosphorus compared with preovulatory follicles. These results indicated the marked differences in follicular fluid composition between preovulatory and cystic follicles in buffalo. Some of the changes were indicative of oxidative stress and disturbed steroidogenesis, two important mechanisms shown to be associated with cystic ovarian disease in various species. Further studies are warranted to investigate whether these differences are directly or indirectly involved in the formation of cystic follicles or are mere manifestations of the condition.
本研究对比分析了水牛（）排卵前卵泡和囊状卵泡的卵泡液的生化、激素和矿物质组成。本研究共从屠宰场随机采集了 215 头水牛的生殖道及完整卵巢。在非繁殖季节，本研究中囊状卵泡的发生率为 3.72%（8/215），其中 62.5%的病例发生在右侧卵巢，37.5%的病例发生在左侧卵巢。从排卵前卵泡（直径 12-15 毫米，雌激素活性，卵泡期或黄体期 IV 期的两个卵巢之一，n = 10）和囊状卵泡（至少 20 毫米直径，两个卵巢之一没有黄体，n = 8）中抽吸卵泡液。对卵泡液样本进行生化成分（尿酸、肌酐、血尿素氮、胆固醇、总蛋白、葡萄糖、抗坏血酸和碱性磷酸酶）、激素（孕酮、雌二醇和胰岛素）和矿物质（钙、镁、磷、铜、锌和钴）分析。囊状卵泡的肌酐、血尿素氮、胆固醇、孕酮、铜、锌和钴浓度较高（<0.05），尿酸、葡萄糖、抗坏血酸、雌二醇、胰岛素、钙、镁和磷浓度较低（<0.05）与排卵前卵泡相比。这些结果表明，水牛排卵前卵泡和囊状卵泡的卵泡液成分存在明显差异。一些变化表明存在氧化应激和类固醇生成障碍，这两种重要机制已被证明与多种物种的囊状卵巢疾病有关。需要进一步的研究来探讨这些差异是否直接或间接参与囊状卵泡的形成，或者仅仅是该疾病的表现。
