Animal Reproduction Division, Indian Veterinary Research Institute, Izatnagar, India.
Anim Reprod Sci. 2011 Aug;127(1-2):38-42. doi: 10.1016/j.anireprosci.2011.07.013. Epub 2011 Jul 30.
This study describes the changes in biochemical composition of follicular fluid during reproductive acyclicity in buffalo. A total of 73 pairs of ovaries collected from 26 reproductively acyclic and 47 reproductively cyclic buffaloes were used in the investigation. Ovarian follicles were classified into small (5.0-6.9 mm), medium (7.0-9.9 mm) and large (≥10.0 mm) sized categories depending upon their diameter. Follicular fluid was aspirated, processed and assayed for glucose, cholesterol, total protein, acid phosphatase and alkaline phosphatase. Glucose concentration was lesser in reproductively acyclic compared to cyclic buffaloes (19.3 ± 2.59 mg/dl compared to 32.6 ± 2.60 mg/dl; P<0.05), mainly due to difference in concentration between small sized follicles (12.4 ± 2.59 mg/dl compared to 28.0 ± 3.32 mg/dl; P<0.05). Cholesterol concentration was also lesser in reproductively acyclic compared to cyclic buffaloes (32.2 ± 2.14 mg/dl compared to 35.5 ± 2.16 mg/dl; P<0.05) and this was related to the lesser concentration found in large follicles (13.8 ± 3.45 mg/dl compared to 37.2 ± 4.10mg/dl; P<0.001). Total protein and acid phosphatase levels were not affected by either the reproductive cyclicity status or the follicular size (4.9 ± 1.07 g/dl to 6.0 ± 0.28 g/dl and 1.2 ± 0.17 U/dl to 2.5 ± 1.22 U/dl, respectively). An increased alkaline phosphatase activity was, however, observed in reproductively acyclic compared to cyclic buffaloes (27.5 ± 3.08 U/dl compared to 14.0 ± 1.09 U/dl; P<0.0001). In conclusion, results of the present study indicate an alteration in the biochemical composition of follicular fluid during reproductive acyclicity in buffalo. The findings provide further support to the notion that poor nutrition is an important factor triggering reproductive acyclicity in buffalo.
本研究描述了水牛繁殖周期中非周期性卵巢中滤泡液生化成分的变化。本研究共使用了 26 只非周期性和 47 只周期性繁殖的水牛的 73 对卵巢。根据其直径，将卵巢滤泡分为小（5.0-6.9 毫米）、中（7.0-9.9 毫米）和大（≥10.0 毫米）尺寸类别。抽吸、处理并测定滤泡液中的葡萄糖、胆固醇、总蛋白、酸性磷酸酶和碱性磷酸酶。非周期性繁殖的水牛的葡萄糖浓度（19.3 ± 2.59 mg/dl）低于周期性繁殖的水牛（32.6 ± 2.60 mg/dl；P<0.05），主要是由于小滤泡（12.4 ± 2.59 mg/dl）之间的浓度差异造成的与 28.0 ± 3.32 mg/dl；P<0.05）。非周期性繁殖的水牛的胆固醇浓度（32.2 ± 2.14 mg/dl）也低于周期性繁殖的水牛（35.5 ± 2.16 mg/dl；P<0.05），这与大滤泡中发现的较低浓度有关（13.8 ± 3.45 mg/dl 与 37.2 ± 4.10mg/dl；P<0.001）。总蛋白和酸性磷酸酶水平不受生殖周期性状态或滤泡大小的影响（分别为 4.9 ± 1.07 g/dl 至 6.0 ± 0.28 g/dl 和 1.2 ± 0.17 U/dl 至 2.5 ± 1.22 U/dl）。然而，在非周期性繁殖的水牛中观察到碱性磷酸酶活性增加（27.5 ± 3.08 U/dl 与 14.0 ± 1.09 U/dl；P<0.0001）。总之，本研究结果表明，在水牛繁殖周期中非周期性卵巢中，滤泡液的生化成分发生了变化。这些发现进一步支持了营养不良是触发水牛繁殖周期非周期性的一个重要因素的观点。
