Graduate Program in Food and Health (Programa de Pós-Graduação em Alimentos e Saúde - PPGAS, Federal University of Minas Gerais (Universidade Federal de Minas Gerais - UFMG), Montes Claros, Minas Gerais, Brazil.
Graduate Program in Health Sciences (Programa de Pós-Graduação em Ciências da Saúde - PPGCS), State University of Montes Claros (Universidade Estadual de Montes Claros - Unimontes), Montes Claros, Minas Gerais, Brazil.
Lasers Med Sci. 2023 Mar 15;38(1):85. doi: 10.1007/s10103-023-03743-z.
To evaluate the effects of Light-Emitting Diode (LED) irradiation on the expression of thermogenesis and lipogenesis-associated markers in adipose tissue and metabolic parameters of obese mice. Twenty-four male mice were divided into four groups: i) ST fed standard diet; ii) HCD fed hyperglycemic diet; iii) LED + I fed hyperglycemic diet and irradiated with LED in the interscapular region; iv) LED + A fed hyperglycemic diet and irradiated with LED in the abdominal region. The first phase of the study comprehended the induction of obesity for 12 weeks. Next, the animals were submitted to six irradiation sessions (days 1, 3, 7, 10, 14, and 21) using a 660-nm LED (5.77 J/cm at 48,1 mW/cm). Anthropometric, biochemical, and histological parameters and the expression of thermogenesis and lipogenesis-associated markers were assessed in adipose tissue. There was diminished weight gain between the HCD and LED + A groups (ST: 0.37 ± 0.65; HCD: 3.10 ± 0.89; LED + I: -1.26 ± 0.83; LED + A: -2.07 ± 1.27 g; p < 0.018). There was a 33.3% and 23.8% reduction in epidydimal adipose tissue weight and a 25% and 10.7% in the visceral adiposity for the LED + I and LED + A groups, respectively, when compared with HCD. There was a decreased accumulation of fat droplets in adipose tissue in LED + A and LED + I groups. Additionally, LED irradiation was associated with increased mRNA expression of uncoupling protein 1 (UCP1) in the brown adipose tissue (ST: 2.27 ± 0.19; HCD: 1.54 ± 0.12; LED + I: 2.44 ± 0.22; p = 0.014) and decreased fatty acid synthetase (FAS) expression in epidydimal adipose tissue (ST: 0.79 ± 0.13; HCD: 1.59 ± 0.13; LED + A: 0.85 ± 0.04; p = 0.0008). LED treatment improved anthropometric parameters, possibly associated with the histological alterations, thermogenesis and lipogenesis markers in white adipose tissue, and expression modulation in brown adipose tissue.
评估发光二极管（LED）照射对肥胖小鼠脂肪组织中与产热和脂肪生成相关标记物的表达以及代谢参数的影响。
将 24 只雄性小鼠分为四组：i）标准饮食喂养组（ST）；ii）高糖饮食喂养组（HCD）；iii）高糖饮食喂养并在肩胛间区接受 LED 照射组（LED + I）；iv）高糖饮食喂养并在腹部接受 LED 照射组（LED + A）。研究的第一阶段包括诱导肥胖 12 周。然后，对动物进行六次照射（第 1、3、7、10、14 和 21 天），使用 660nm LED（48,1mW/cm 时为 5.77J/cm）。评估脂肪组织中的体重、生化和组织学参数以及与产热和脂肪生成相关的标记物的表达。HCD 组和 LED + A 组的体重增加明显减少（ST：0.37 ± 0.65；HCD：3.10 ± 0.89；LED + I：-1.26 ± 0.83；LED + A：-2.07 ± 1.27g；p<0.018）。与 HCD 组相比，LED + I 组和 LED + A 组附睾脂肪组织重量分别减少 33.3%和 23.8%，内脏脂肪组织减少 25%和 10.7%。LED + A 组和 LED + I 组脂肪组织中脂肪滴的积累减少。此外，LED 照射与棕色脂肪组织中解偶联蛋白 1（UCP1）mRNA 表达的增加相关（ST：2.27 ± 0.19；HCD：1.54 ± 0.12；LED + I：2.44 ± 0.22；p=0.014），附睾脂肪组织中脂肪酸合成酶（FAS）表达减少（ST：0.79 ± 0.13；HCD：1.59 ± 0.13；LED + A：0.85 ± 0.04；p=0.0008）。LED 治疗改善了人体测量参数，这可能与白色脂肪组织的组织学改变、产热和脂肪生成标记物以及棕色脂肪组织中表达的调节有关。
