假型病毒用于沙粒病毒属。

Pseudotyped Viruses for Mammarenavirus.

机构信息

Jiangsu Recbio Technology Co., Ltd., Taizhou, China.

Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.

出版信息

Adv Exp Med Biol. 2023;1407:279-297. doi: 10.1007/978-981-99-0113-5_15.

DOI:10.1007/978-981-99-0113-5_15

PMID:36920703

Abstract

Mammarenaviruses are classified into New World arenaviruses (NW) and Old World arenaviruses (OW). The OW arenaviruses include the first discovered mammarenavirus-lymphocytic choriomeningitis virus (LCMV) and the highly lethal Lassa virus (LASV). Mammarenaviruses are transmitted to human by rodents, resulting in severe acute infections and hemorrhagic fever. Pseudotyped viruses have been widely used as a tool in the study of mammarenaviruses. HIV-1, SIV, FIV-based lentiviral vectors, VSV-based vectors, MLV-based vectors, and reverse genetic approaches have been applied in the construction of pseudotyped mammarenaviruses. Pseudotyped mammarenaviruses are commonly used in receptor research, neutralizing antibody detection, inhibitor screening, viral virulence studies, functional analysis of N-linked glycans, and studies of viral infection, endocytosis, and fusion mechanisms.

摘要

沙粒病毒科分为新世界沙粒病毒（NW）和旧世界沙粒病毒（OW）。OW 沙粒病毒包括最早发现的沙粒病毒-淋巴细胞性脉络丛脑膜炎病毒（LCMV）和高致死性的拉萨病毒（LASV）。沙粒病毒通过啮齿动物传播给人类，导致严重的急性感染和出血热。假型病毒已被广泛用作沙粒病毒研究的工具。基于 HIV-1、SIV、FIV 的慢病毒载体、基于 VSV 的载体、基于 MLV 的载体和反向遗传方法已被应用于假型沙粒病毒的构建。假型沙粒病毒常用于受体研究、中和抗体检测、抑制剂筛选、病毒毒力研究、N 连接糖基的功能分析以及病毒感染、内吞和融合机制的研究。