葡萄牙即食沙拉中 和 菌属的分子研究。 （注：原文中“for and spp.”部分有缺失信息，这里只能按现有内容翻译）
Molecular investigation of ready-to-eat salads for and spp. in Portugal.
作者信息
Faria Clarissa Perez, Pereira Adelaide, Almeida Daniela, Pinto Miguel, Lourenço Ágata, do Céu Sousa Maria
机构信息
CNC - Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, 3030-548 Coimbra, Portugal.
Faculty of Pharmacy, University of Coimbra, Azinhaga de Santa Comba, 3030-548 Coimbra, Portugal.
出版信息
Food Waterborne Parasitol. 2023 Feb 23;30:e00190. doi: 10.1016/j.fawpar.2023.e00190. eCollection 2023 Mar.
Foodborne outbreaks are often associated with the consumption of salads. However, published studies on the detection of foodborne pathogens in ready-to-eat salads are scarce. The aim of this study was to detect and DNA in ready-to-eat salads, by applying techniques of molecular biology to study the frequency of contamination in salads. A total of 100 packages of ready-to-eat salads containing assorted leafy green vegetables were randomly purchased from hypermarkets located in central regions of Portugal (Coimbra and Viseu). Nested-PCR and qPCR methods were used to detect and DNA. Species and assemblages of the parasites were identified by sequence analysis and PCR. Eighteen of the 100 samples (18%) were positive for and twelve were sequenced and identified as assemblage A. spp. were not detected in any salads. Overall, pre-harvest and post-harvest preventive measures may be need for control throughout the food production industry, from the field to consumers.
食源性疾病暴发通常与食用沙拉有关。然而，关于即食沙拉中食源性病原体检测的已发表研究很少。本研究的目的是通过应用分子生物学技术来检测即食沙拉中的[病原体名称]DNA，以研究沙拉中的污染频率。从葡萄牙中部地区（科英布拉和维塞乌）的大型超市随机购买了100包含有各种叶菜类蔬菜的即食沙拉。采用巢式PCR和qPCR方法检测[病原体名称]DNA。通过序列分析和PCR鉴定寄生虫的种类和组合。100个样本中有18个（18%）[病原体名称]呈阳性，其中12个进行了测序并鉴定为组合A。在任何沙拉中均未检测到[另一种病原体名称]。总体而言，从田间到消费者，整个食品生产行业可能需要采取收获前和收获后的预防措施来控制[病原体名称]。
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