The cuticle plays an important role against toxicity to bisphenol A and bisphenol S.

represents a favorite non-mammalian animal model, which is often used to study the effect of foreign substances on living organisms. Its epidermal barrier is a primary biological barrier that protects nematodes from the toxicity of chemicals. In this study, we investigated the effect of Bisphenol A (BPA), an endocrine disrupting chemical, and its structural analog Bisphenol S (BPS), which is often used as a substitute for BPA in some products, on the behavior of wild type (N2) and mutant strain, which is characterized by the production of abnormal cuticle blisters. We found that exposure of wild type (N2), as well as its mutant strain , to selected concentrations of BPA (0.1, 0.5, 1 and 5 µM) and BPS (0.1, 0.5, 1 and 5 µM) resulted in significant changes in reproduction, habituation behavior, and body length of nematodes. Based on our findings, we can conclude that BPS, which was supposed to be a safer alternative to BPA, caused almost identical detrimental effects on behavior. Furthermore, compared to the wild type of , these effects were more pronounced in the strain, which is characterized by a mutation in an individual collagen gene responsible for proper cuticle formation, underlying the role of the epidermal barrier in bisphenol toxicity. Taken together, our data indicate the potential risks of using BPS as a BPA alternative.