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人多能干细胞来源的脑类器官中神经干细胞增殖分析的优化方案。

Optimized protocol for analysis of neural stem proliferation in human-pluripotent-stem-cell-derived cerebral organoids.

作者信息

Tang Xiao-Yan, Wang Da, Zhang Xin-Yue, Xu Min, Liu Yan

机构信息

Institute for Stem Cell and Neural Regeneration, School of Pharmacy; State Key Laboratory of Reproductive Medicine; Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine; Nanjing Medical University, Nanjing, China.

Institute for Stem Cell and Neural Regeneration, School of Pharmacy; State Key Laboratory of Reproductive Medicine; Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine; Nanjing Medical University, Nanjing, China.

出版信息

STAR Protoc. 2023 Mar 14;4(2):102169. doi: 10.1016/j.xpro.2023.102169.

DOI:10.1016/j.xpro.2023.102169
PMID:36924505
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10026034/
Abstract

Cerebral organoids represent an optimal experimental system for studying human cortical development, evolution, physiology, function, and disease mechanisms. Here, we describe a simple protocol for the differentiation of human pluripotent stem cells (hPSCs) into cerebral organoids. We describe steps for hPSC maintenance, neural induction of embryoid bodies, and patterning of cerebral organoids. We also detail a process for the phenotypic assay of each neural-tube-like area in hPSC-derived cerebral organoids. For complete details on the use and execution of this protocol, please refer to Tang et al. (2021)..

摘要

大脑类器官是研究人类皮质发育、进化、生理学、功能和疾病机制的理想实验系统。在此，我们描述了一种将人类多能干细胞（hPSC）分化为大脑类器官的简单方案。我们阐述了hPSC维持、胚状体的神经诱导以及大脑类器官模式形成的步骤。我们还详细介绍了hPSC来源的大脑类器官中每个神经管样区域的表型分析过程。有关该方案使用和执行的完整详细信息，请参考Tang等人（2021年）的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/568590edd546/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/05c43b937378/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/60c96efdca4c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/a82f069f5edf/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/04b50b67f17f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/f979eac6f9b2/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/0fabbedf5435/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/c48dfe8dc08b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/568590edd546/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/05c43b937378/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/60c96efdca4c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/a82f069f5edf/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/04b50b67f17f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/f979eac6f9b2/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/0fabbedf5435/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/c48dfe8dc08b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82bf/10026034/568590edd546/gr7.jpg

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Human neural tube morphogenesis in vitro by geometric constraints.体外通过几何约束进行人体神经管形态发生。
Nature. 2021 Nov;599(7884):268-272. doi: 10.1038/s41586-021-04026-9. Epub 2021 Oct 27.
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DSCAM/PAK1 pathway suppression reverses neurogenesis deficits in iPSC-derived cerebral organoids from patients with Down syndrome.
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Artificial Meshed Vessel-Induced Dimensional Breaking Growth of Human Brain Organoids and Multiregional Assembloids.人工网状血管诱导人脑类器官和多区域组装体的尺寸突破生长
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Correspondence to "Bipolar disorder-iPSC derived neural progenitor cells exhibit dysregulation of store-operated Ca entry and accelerated differentiation" by Hewitt et al. (PMID: 37402854).致休伊特等人的《双相情感障碍——诱导多能干细胞衍生的神经祖细胞表现出储存式钙内流失调和加速分化》（ PMID：37402854）
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