Laboratorio de Biotecnología, Universidad ORT Uruguay, Mercedes 1237, 11100 Montevideo, Uruguay; Graduate Program in Chemistry, Facultad de Química, Universidad de la República, Uruguay.
Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, UANL, San Nicolás de los Garza, Mexico; Centro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, Mexico.
Biotechnol Adv. 2023 Jul-Aug;65:108127. doi: 10.1016/j.biotechadv.2023.108127. Epub 2023 Mar 15.
Different from other aerobic microorganisms that oxidise carbon sources to water and carbon dioxide, Gluconobacter catalyses the incomplete oxidation of various substrates with regio- and stereoselectivity. This ability, as well as its capacity to release the resulting products into the reaction media, place Gluconobacter as a privileged member of a non-model microorganism class that may boost industrial biotechnology. Knowledge of new technologies applied to Gluconobacter has been piling up in recent years. Advancements in its genetic modification, application of immobilisation tools and careful designs of the transformations, have improved productivities and stabilities of Gluconobacter strains or enabled new bioconversions for the production of valuable marketable chemicals. In this work, the latest advancements applied to Gluconobacter-catalysed biotransformations are summarised with a special focus on recent available tools to improve them. From genetic and metabolic engineering to bioreactor design, the most recent works on the topic are analysed in depth to provide a comprehensive resource not only for scientists and technologists working on/with Gluconobacter, but for the general biotechnologist.
不同于其他需将碳源氧化为水和二氧化碳的需氧微生物，葡萄糖酸杆菌具有区域和立体选择性，能够催化各种底物的不完全氧化。这种能力以及将产物释放到反应介质中的能力，使葡萄糖酸杆菌成为可能推动工业生物技术发展的非模式微生物类的特权成员。近年来，应用于葡萄糖酸杆菌的新技术知识不断积累。其遗传修饰、固定化工具的应用以及转化的精心设计，提高了葡萄糖酸杆菌菌株的生产力和稳定性，或实现了新的生物转化，以生产有价值的商品化学品。在这项工作中，总结了最新应用于葡萄糖酸杆菌催化生物转化的进展，特别关注了最近可用的改进工具。从遗传和代谢工程到生物反应器设计，对该主题的最新研究工作进行了深入分析，不仅为从事/使用葡萄糖酸杆菌的科学家和技术人员提供了全面的资源，也为一般生物技术人员提供了资源。
