Zhang Liwen, Zhang Zheling, Xu Sunan, Zhang Xiaojun, Liu Xiaodan
College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Fish Shellfish Immunol. 2023 Apr;135:108692. doi: 10.1016/j.fsi.2023.108692. Epub 2023 Mar 15.
Non-O1 Vibrio cholerae, a member of the Vibrio family, could cause gastrointestinal infection of Macrobrachium rosenbergii and result in significant economic losses. However, few studies on microRNA immunity related to non-O1 V. cholerae infection of M. rosenbergii. The aim of this study was to elucidate the mechanism of miRNA in the potential immune response of M. rosenbergii. to non-O1 V. cholerae MSVC-GY01 infection by transcriptome sequencing. Following quality screening, the control group received 10, 616, 712 clean reads, whereas the infected group received 9,727,616. The miRNA sequences in the two samples are extremely consistent and have a length of roughly 23 nt. In all, 871 known miRNAs were discovered, with 279 differentially expressed miRNAs (DEMs). Meanwhile, 62 novel miRNAs were predicted, including 43 DEMs. In order to understand the immune-related biological functions of DEMs, target genes were predicted. Pathway function annotation analysis showed that non-O1 V. cholerae affected the NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, and Toll-like receptor signaling pathway, suggesting that miRNAs in the hepatopancreas play a key role in immune responses to pattern recognition receptors. Twelve DEMs were randomly selected for Quantitative Real-time PCR (qRT-PCR). Overall, the expression trends of qRT-PCR were consistent with the sequencing results. These findings corroborate the immunomodulatory function of miRNA in M. rosenbergii against non-O1 V. cholerae infection and provide guidance for the prevention and treatment of related illnesses.
非O1群霍乱弧菌是弧菌属的成员之一，可引起罗氏沼虾的胃肠道感染并导致重大经济损失。然而，关于罗氏沼虾非O1群霍乱弧菌感染相关的微小RNA免疫的研究很少。本研究的目的是通过转录组测序阐明微小RNA在罗氏沼虾对非O1群霍乱弧菌MSVC-GY01感染的潜在免疫反应中的作用机制。经过质量筛选，对照组获得10,616,712条clean reads，而感染组获得9,727,616条。两个样本中的微小RNA序列极其一致，长度约为23 nt。总共发现了871个已知的微小RNA，其中有279个差异表达的微小RNA（DEM）。同时，预测了62个新的微小RNA，包括43个DEM。为了了解DEM的免疫相关生物学功能，对靶基因进行了预测。通路功能注释分析表明，非O1群霍乱弧菌影响了NOD样受体信号通路、RIG-I样受体信号通路和Toll样受体信号通路，这表明肝胰腺中的微小RNA在对模式识别受体的免疫反应中起关键作用。随机选择12个DEM进行定量实时PCR（qRT-PCR）分析。总体而言，qRT-PCR的表达趋势与测序结果一致。这些发现证实了微小RNA在罗氏沼虾抵抗非O1群霍乱弧菌感染中的免疫调节功能，并为相关疾病的预防和治疗提供了指导。
