Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Guangzhou, Guangdong, China.
Guangzhou SoonHeal Medical Technology Co., Ltd., Guangzhou 510000, China.
Biomater Sci. 2023 May 2;11(9):3214-3226. doi: 10.1039/d2bm02063g.
The severe pain caused by oral ulcers seriously affects food intake and speech, bringing great inconvenience in daily life. Drug-loaded patches are mostly used to treat oral mucosal diseases such as oral ulcers and oral lichen planus, but their effects are limited because of the influences of saliva and muscle movement. To enhance the adhesion of drug-loaded patches used in the oral cavity, we designed antimicrobial peptides (AMPs)-modified polycaprolactone (PCL)-collagen nanosheets (APCNs). The internal layer is a bioactive and antibacterial collagen layer modified with antimicrobial peptides. The backing layer is a hydrophobic PCL layer with good mechanical strength that can reduce external influences. We have characterized and tested the APCNs. First, the APCNs exhibited continuous and strong adhesion to irregular buccal mucosa surfaces under wet conditions and external force action. Antibacterial experiments showed that the APCNs had high antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria. Moreover, the APCNs showed good biocompatibility and promoted the adhesion of fibroblasts . Furthermore, APCNs treatment accelerated ulcer healing in a Sprague Dawley rat oral ulcer model. Our study developed antibacterial, wet-adhesive, and healing-promoting PCL-collagen nanosheets and demonstrated that these nanosheets could be promising adhesive therapeutic agents for the treatment of oral mucosal ulcers.
口腔溃疡引起的剧烈疼痛严重影响进食和言语，给日常生活带来极大不便。载药贴剂主要用于治疗口腔溃疡、口腔扁平苔藓等口腔黏膜疾病，但由于唾液和肌肉运动的影响，其效果有限。为了增强口腔载药贴剂的黏附性，我们设计了抗菌肽（AMPs）修饰的聚己内酯（PCL）-胶原蛋白纳米片（APCNs）。内层是经抗菌肽修饰的具有生物活性和抗菌性的胶原蛋白层，外层是具有良好机械强度的疏水性 PCL 层，可减少外部影响。我们对 APCNs 进行了表征和测试。首先，APCNs 在湿润条件下和外力作用下对不规则的颊黏膜表面表现出连续且强大的黏附力。抗菌实验表明，APCNs 对革兰氏阳性菌和革兰氏阴性菌均具有高抗菌活性。此外，APCNs 表现出良好的生物相容性并促进成纤维细胞的黏附。进一步的，APCNs 处理加速了 Sprague Dawley 大鼠口腔溃疡模型中的溃疡愈合。我们的研究开发了具有抗菌、湿黏附性和促进愈合作用的 PCL-胶原蛋白纳米片，并证明这些纳米片可能成为治疗口腔黏膜溃疡的有前途的黏附治疗剂。
