Xuan Chengkai, Hao Lijin, Liu Xuemin, Zhu Ye, Yang Huishang, Ren Yipeng, Wang Lin, Fujie Toshinori, Wu Hongkai, Chen Yunhua, Shi Xuetao, Mao Chuanbin
School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China.
Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, 101 Stephenson Parkway, Norman, OK, 73019-5300, USA.
Biomaterials. 2020 Sep;252:120018. doi: 10.1016/j.biomaterials.2020.120018. Epub 2020 Apr 15.
Healing soft-tissue wounds with an irregular, complicated topography in a bleeding environment demands the development of a dressing that is wet-adhesive, haemostatic, and antibacterial. To meet this unmet demand, we designed a flexible nanosheet (~77 nm thick) made of two layers, one is the antibacterial and haemostatic gelatin modified with dopamine (DA) and antimicrobial peptide (AMP) and mixed with Ca ions as coagulation factors, and another is the mechanically strong polycaprolactone (PCL). This flexible nanosheet exhibited robust mechanical strength, continuous and effective adhesion to a topographically irregular tissue surface under a wet condition, and a high platelet adhesion capacity. Moreover, the nanosheet presented a significantly reduced clotting time of 4 min and a high bactericidal rate of nearly 100%. An in vivo evaluation of the nanosheet using both murine dorsal skin and liver models further revealed that the nanosheet could successfully seal and heal the wounds in a bleeding environment, efficiently control haemorrhaging, and exert an excellent antibacterial effect in two weeks. Our work suggests that this nanosheet holds great promise in healing the bleeding soft-tissue wounds for treating acute trauma.
在出血环境中愈合具有不规则、复杂形貌的软组织伤口需要开发一种具有湿粘性、止血性和抗菌性的敷料。为满足这一未被满足的需求，我们设计了一种由两层组成的柔性纳米片（约77纳米厚），一层是用多巴胺（DA）和抗菌肽（AMP）修饰并与钙离子作为凝血因子混合的抗菌止血明胶，另一层是机械强度高的聚己内酯（PCL）。这种柔性纳米片表现出强大的机械强度，在潮湿条件下对形貌不规则的组织表面具有持续有效的粘附力，以及高血小板粘附能力。此外，该纳米片的凝血时间显著缩短至4分钟，杀菌率高达近100%。使用小鼠背部皮肤和肝脏模型对该纳米片进行的体内评估进一步表明，该纳米片能够在出血环境中成功封闭并愈合伤口，有效控制出血，并在两周内发挥出色的抗菌效果。我们的工作表明，这种纳米片在治疗急性创伤的出血性软组织伤口方面具有巨大潜力。
