Alfaro-Mora Ramsés, Castro-Vásquez Ruth, Rodríguez Julia, Dolz Gaby
Universidad Nacional, Posgrado Regional en Ciencias Veterinarias Tropicales, Maestría en Enfermedades Tropicales, Heredia, Costa Rica.
Dirección de Investigación, Universidad Latina de Costa Rica, San José, Costa Rica.
Arch Virol. 2023 Mar 16;168(4):114. doi: 10.1007/s00705-023-05745-2.
Bovine papillomaviruses (BPVs) infect the basal layer of the epithelium of bovines, where they persist asymptomatically or produce benign fibroepithelial hyperplasia in the skin or mucosa. The aim of the present study was to describe the genotypes of bovine papillomas at the macroscopic and microscopic level. A descriptive study was carried out using non-probabilistic convenience sampling. Ninety-nine papillomas from 63 animals were collected on 32 farms, as well as information about age, gender, breed, and productive use of the bovines. The location, type, and degree of epithelial invasion of the papillomas were recorded. The samples were subjected to molecular and histopathological analysis. Papillomas were found most frequently on dairy farms (75.0%), in females (95.0%), in cattle of the Holstein breed (45.0%), and in animals over 24 months of age (50.0%). Most of the positive animals had from 1 to 15 papillomas (31.6%) and only one type of papilloma (79.4%). Cauliflower-like papillomas were found in 48.5% of the cases, while atypical papillomas were found in 11.1% of the cases. Cauliflower-like papillomas were found mainly on the udder (14.4%), head (10.0%), and neck (10.0%) and were associated with five BPV genotypes (BPV1, BPV2, BPV6, BPV7, and BPV10), while BPV2 and BPV6 were found to be associated with all types of papillomas (cauliflower, flat, pedunculated, and atypical). The presence of BPV11 in flat papillomas and BPV6 in atypical papillomas is reported here for the first time. Morphology and histopathological findings did not allow differentiation of the BPV genotypes.
牛乳头瘤病毒（BPVs）感染牛的上皮基底层，在该部位它们可无症状持续存在，或在皮肤或黏膜产生良性纤维上皮增生。本研究的目的是在宏观和微观层面描述牛乳头瘤的基因型。采用非概率便利抽样进行了一项描述性研究。从32个农场的63只动物身上收集了99个乳头瘤，以及有关牛的年龄、性别、品种和生产用途的信息。记录了乳头瘤的位置、类型和上皮侵袭程度。对样本进行了分子和组织病理学分析。乳头瘤最常见于奶牛场（75.0%）、雌性动物（95.0%）、荷斯坦品种的牛（45.0%）以及24个月龄以上的动物（50.0%）。大多数阳性动物有1至15个乳头瘤（31.6%），且只有一种类型的乳头瘤（79.4%）。48.5%的病例中发现了菜花状乳头瘤，11.1%的病例中发现了非典型乳头瘤。菜花状乳头瘤主要见于乳房（14.4%）、头部（10.0%）和颈部（10.0%），并与五种BPV基因型（BPV1、BPV2、BPV6、BPV7和BPV10）相关，而BPV2和BPV6与所有类型的乳头瘤（菜花状、扁平状、带蒂状和非典型性）均相关。本文首次报道扁平乳头瘤中存在BPV11以及非典型乳头瘤中存在BPV6。形态学和组织病理学结果无法区分BPV基因型。
