基于 1 基因扩增对波兰南部外阴阴道假丝酵母菌病患者中密切相关种的流行情况分析。
Prevalence of Closely Related Species among Patients with Vulvovaginal Candidiasis in Southern Poland Based on the 1 Gene Amplification.
机构信息
Department of Pharmaceutical Microbiology, Jagiellonian University Medical College, Cracow, Poland.
Centre of Microbiological Research and Autovaccines, Cracow, Poland.
出版信息
Pol J Microbiol. 2023 Mar 24;72(1):69-77. doi: 10.33073/pjm-2023-011. Print 2023 Mar 1.
remains the most common species isolated from women with vulvovaginal candidiasis. However, closely related species such as and may also occur, although they are often misidentified. The aim of the study was to confirm the phenotypic identification of and its closely related species isolated from women with genital tract infections by amplification of the 1 (hyphal wall protein 1) gene in a PCR assay. We report a detailed molecular identification of and its closely related species among 326 patients in the Małopolska region, Poland. Initial phenotypic identifications were confirmed by amplification of the 1 gene. Based on molecular analysis, we revealed 307 strains (94.17%) as and 17 as (5.22%). No strain of was detected. Two patients h ad co-infection with and (0.61%). A PCR assay targeting the 1 gene was reliable for correctly identifying species among the complex.
仍然是从患有外阴阴道念珠菌病的女性中分离出来的最常见的物种。然而，也可能存在密切相关的物种，如 和 ，尽管它们经常被错误识别。本研究的目的是通过聚合酶链反应（PCR）试验扩增 1（菌丝壁蛋白 1）基因，确认从患有生殖道感染的女性中分离出的 及其密切相关的种的表型鉴定。我们报告了波兰小波兰地区 326 例患者中 的详细分子鉴定及其密切相关的种。通过扩增 1 基因来确认初始表型鉴定。基于分子分析，我们发现 307 株（94.17%）为 ，17 株为 （5.22%）。未检测到 的菌株。两名患者同时感染 和 （0.61%）。针对 1 基因的 PCR 检测对于正确鉴定 复合体中的种是可靠的。
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