Sharma R, Bulmer D, Peel S
University of Southampton.
J Anat. 1986 Feb;144:189-99.
The numbers of granulated metrial gland cells, the percentage of them incorporating tritiated thymidine and the numbers of granulated metrial gland cell precursors in a defined area of the metrial gland, and the cross sectional area of the metrial gland, have been determined in mice ovariectomised on Day 8 of pregnancy. The effects, on these parameters and on fetal survival, of progesterone treatment, given at two levels (0.5 mg or 1 mg twice daily) and started immediately after ovariectomy or after 6, 10 or 24 hours delay, have been studied. Granulated metrial gland cell number, DNA synthetic activity and metrial gland size decreased rapidly after ovariectomy but there was a significant increase in the number of granulated metrial gland cell precursors in the first 24 hours. Progesterone treatment, at the higher dose level started immediately after ovariectomy, resulted in significantly more granulated metrial gland cells in the area analysed two days after ovariectomy although a normal proportion of them were in DNA synthesis. Immediate progesterone treatment, at the higher dose level, was able to prolong the DNA synthetic activity of granulated metrial gland cells until Day 19 and this probably accounted for the significantly greater numbers of granulated metrial gland cells in these mice than in control mice, at this time. Delaying progesterone treatment at the higher level by 24 hours significantly reduced the size of the metrial glands at Day 10 but there was no effect on the cellular composition of the area analysed. Delaying the lower dose of progesterone treatment by 24 hours also resulted in small metrial glands and was associated with significantly fewer granulated metrial gland cells in the area analysed but significantly more of them were synthesising DNA. The observations are discussed and an attempt is made to relate the effects on individual fetal and placental survival to the effects on the corresponding metrial glands.
已测定了妊娠第8天切除卵巢的小鼠子宫蜕膜腺特定区域内颗粒化蜕膜腺细胞的数量、掺入氚标记胸腺嘧啶核苷的细胞百分比、颗粒化蜕膜腺细胞前体的数量以及子宫蜕膜腺的横截面积。研究了在两种剂量水平（每日两次，每次0.5毫克或1毫克）下进行孕酮治疗，并在卵巢切除术后立即开始或在延迟6、10或24小时后开始，对这些参数以及胎儿存活率的影响。卵巢切除术后，颗粒化蜕膜腺细胞数量、DNA合成活性和子宫蜕膜腺大小迅速下降，但在最初24小时内颗粒化蜕膜腺细胞前体数量显著增加。卵巢切除术后立即开始使用较高剂量水平的孕酮治疗，在卵巢切除术后两天分析的区域内，颗粒化蜕膜腺细胞数量显著增多，尽管其中正常比例的细胞处于DNA合成状态。较高剂量水平的孕酮立即治疗能够将颗粒化蜕膜腺细胞的DNA合成活性延长至第19天，这可能解释了此时这些小鼠中颗粒化蜕膜腺细胞数量比对照小鼠显著更多的原因。将较高剂量的孕酮治疗延迟24小时，显著降低了第10天子宫蜕膜腺的大小，但对分析区域的细胞组成没有影响。将较低剂量的孕酮治疗延迟24小时也导致子宫蜕膜腺较小，并且与分析区域内颗粒化蜕膜腺细胞数量显著减少相关，但其中合成DNA的细胞显著增多。对这些观察结果进行了讨论，并尝试将对个体胎儿和胎盘存活的影响与对相应子宫蜕膜腺的影响联系起来。
