Bulmer D, Peel S
J Anat. 1979 Jan;128(Pt 1):185-94.
A study was made of the changes in morphology and in nuclear incorporation of tritiated thymidine in the rat uterus and placenta after ovariectomy at day 10 of gestation. There was some individual variation between different animals in the results of the ovariectomy, but in general the effects on both maternal and fetal tissues were more severe than those reported to follow ovariectomy at later stages of pregnancy. Even in the animals where there was extensive placental survival 2 or 3 days after the ovariectomy, normal differentiation of the placental labyrinth did not occur and the fetuses failed to survive. Ovariectomy had a pronounced effect on the proliferative activity of uterine epithelial cells. The high labelling index in the control animals up to day 12 was markedly reduced in the ovariectomized animals; after day 13, however, the labelling index of the controls was reduced to a level lower than that of the ovariectomized animals. Nuclear labelling occurred in the glandular epithelium from two days after ovariectomy, but was never present in the controls. There was a marked reduction in the percentage of labelled nuclei in the uterine muscle and in the metrial gland after ovariectomy. In the metrial gland this was associated with a reduction in the number of typical granulated cells and with the appearance of numerous small round cells. It is suggested that the latter represented metrial gland cell precursors which had undergone impairment of their normal differentiation process.
对妊娠第10天进行卵巢切除术后大鼠子宫和胎盘的形态变化以及氚标记胸腺嘧啶核苷的核掺入情况进行了研究。卵巢切除的结果在不同动物之间存在一些个体差异，但总体而言，对母体和胎儿组织的影响比报道的妊娠后期卵巢切除的影响更为严重。即使在卵巢切除术后2或3天仍有广泛胎盘存活的动物中，胎盘迷路也未发生正常分化，胎儿未能存活。卵巢切除对子宫上皮细胞的增殖活性有显著影响。直至第12天，对照动物中的高标记指数在卵巢切除的动物中显著降低；然而，在第13天之后，对照动物的标记指数降至低于卵巢切除动物的水平。卵巢切除术后两天，腺上皮中出现核标记，但对照中从未出现。卵巢切除术后，子宫肌层和蜕膜中标记核的百分比显著降低。在蜕膜中，这与典型颗粒细胞数量的减少以及大量小圆形细胞的出现有关。有人认为，后者代表了正常分化过程受损的蜕膜细胞前体。
