丝状肌动蛋白在活细胞质中的移动性。
Mobility of filamentous actin in living cytoplasm.
作者信息
Wang Y L
机构信息
Doris W. Neustadt Laboratory of Cellular Structure, Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
出版信息
J Cell Biol. 1987 Dec;105(6 Pt 1):2811-6. doi: 10.1083/jcb.105.6.2811.
Abstract
Filamentous actin in living cultured cells was labeled by microinjecting trace amounts of rhodamine-phalloidin (rh-pha) as a specific, high-affinity probe. The microinjection caused no detectable effect on cell morphology or cell division. The distribution of rh-pha-labeled filaments was then examined in dividing cells using image-intensified fluorescence microscopy, and the exchangeability of labeled filaments along stress fibers was studied during interphase using fluorescence recovery after photobleaching. rh-pha showed a rapid concentration at the contractile ring during cell division. In addition, recovery of fluorescence after photobleaching occurred along stress fibers with a halftime as short as 8 min. These observations suggest that at least some actin filaments undergo continuous movement and reorganization in living cells. This dynamic process may play an important role in various cellular functions.
摘要
通过微量注射罗丹明-鬼笔环肽（rh-pha）作为一种特异性高亲和力探针，对活培养细胞中的丝状肌动蛋白进行标记。微量注射对细胞形态或细胞分裂未产生可检测到的影响。然后，使用图像增强荧光显微镜检查分裂细胞中rh-pha标记细丝的分布，并在间期使用光漂白后的荧光恢复研究标记细丝沿应力纤维的交换能力。rh-pha在细胞分裂期间在收缩环处迅速聚集。此外，光漂白后荧光沿着应力纤维恢复，半衰期短至8分钟。这些观察结果表明，至少一些肌动蛋白丝在活细胞中经历持续的运动和重组。这一动态过程可能在各种细胞功能中发挥重要作用。
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本文引用的文献
1
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Eur J Cell Biol. 1981 Jun;24(2):176-83.
2
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Cell Motil. 1982;2(2):103-13. doi: 10.1002/cm.970020203.
3
Structural interaction of cytoskeletal components.细胞骨架成分的结构相互作用。
J Cell Biol. 1981 Jul;90(1):222-35. doi: 10.1083/jcb.90.1.222.
4
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Eur J Cell Biol. 1980 Jun;21(2):188-94.
5
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6
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