School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Sci Rep. 2023 Mar 18;13(1):4504. doi: 10.1038/s41598-023-31660-2.
In this study, a powerful and rapid aqueous two-phase system (ATPS) method was used to extract polysaccharides from Codonopsis pilosula. The ATPS process was investigated with response surface methodology (RSM). At an ammonium sulfate concentration of 17%, ethanol concentration of 30%, and extraction temperature of 40 °C at pH 6, the total extraction yield of polysaccharides reached (31.57 ± 1.28)%. After separation and purification, a homogenized polysaccharide CPP 2-4 with molecular weight of 3.9 × 10 kDa was obtained from the bottom phase. The physicochemical properties and structural features confirmed that CPP 2-4 was an α-1,6-glucan. Activity studies showed that the IC of CPP 2-4 for DPPH radical scavenging was 0.105 mg/mL. The FRAP and ABTS assays showed that CPP 2-4 had strong antioxidant activity in a dose-dependent manner. Furthermore, CPP 2-4 inhibited NO release in RAW264.7 cells induced by lipopolysaccharide, which indicated a certain anti-inflammatory effect. This study improved the extraction rate of polysaccharides from C. pilosula and identified a glucan for the first time, that can contribute to a better understanding of the composition and structure of polysaccharides from C. pilosula and provide data support for the medicine and food homology of C. pilosula.
在这项研究中，采用了一种强大而快速的双水相体系（ATPS）方法从党参中提取多糖。采用响应面法（RSM）研究了 ATPS 工艺。在硫酸铵浓度为 17%、乙醇浓度为 30%、提取温度为 40°C、pH 值为 6 的条件下，多糖的总提取率达到（31.57±1.28）%。经过分离和纯化，从底部相中得到一种均相多糖 CPP 2-4，其分子量为 3.9×10kDa。理化性质和结构特征表明 CPP 2-4 是一种α-1,6-葡聚糖。活性研究表明，CPP 2-4 对 DPPH 自由基清除的 IC 为 0.105mg/mL。FRAP 和 ABTS 测定表明，CPP 2-4 具有较强的抗氧化活性，呈剂量依赖性。此外，CPP 2-4 抑制脂多糖诱导的 RAW264.7 细胞中 NO 的释放，表明其具有一定的抗炎作用。本研究提高了党参多糖的提取率，并首次鉴定出一种葡聚糖，有助于更好地了解党参多糖的组成和结构，并为党参的药食同源性提供数据支持。
