Faculty of Chemistry, University of Warsaw, Pasteura 1 Str., PL 02-093 Warsaw, Poland; Nencki Institute of Experimental Biology of Polish Academy of Sciences, Pasteura 3 St., 02-093 Warsaw, Poland.
Faculty of Chemistry, University of Warsaw, Pasteura 1 Str., PL 02-093 Warsaw, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jul 5;295:122606. doi: 10.1016/j.saa.2023.122606. Epub 2023 Mar 13.
The DNA biosensors are powerful tools in the gene mutation or pathogens detection. That is why there are a lot of DNA detection strategies and methods. Here we present the insight on a slightly overlooked DNA detection technique, surface-enhanced Raman scattering (SERS). The present work is a summary of the influence of the plasmonic metal of the SERS substrate and strategy of the sandwich-type biosensor construction, simply the placement of the Raman reporter and mismatches, on the SERS signal enhancement. We found that, although in general there is an increase in the intensity of the SERS signal when the distance between the Raman scatterer and the SERS-active surface decreases, for this type of DNA SERS sensor a greater intensity of the measured Raman signal is usually observed when the Raman reporter is farther away from the plasmonic substrate. This is probably caused by a significant change in the hybridisation efficiency for the different structures of the sensor analysed due to some steric hindrances.
DNA 生物传感器是基因突变或病原体检测的有力工具。这就是为什么有很多 DNA 检测策略和方法。在这里，我们介绍了一个稍微被忽视的 DNA 检测技术，表面增强拉曼散射（SERS）的深入了解。目前的工作是对 SERS 衬底的等离子体金属和三明治型生物传感器构建策略（即拉曼报告分子和错配的简单放置）对 SERS 信号增强的影响的总结。我们发现，尽管通常当拉曼散射体和 SERS 活性表面之间的距离减小时，SERS 信号的强度会增加，但对于这种类型的 DNA SERS 传感器，当拉曼报告分子远离等离子体衬底时，通常会观察到测量的拉曼信号的强度更大。这可能是由于由于一些空间位阻，分析的传感器的不同结构的杂交效率发生了显著变化。
