Wang Gang, Cheng Tao, Yuan Hongxiu, Zou Fangbo, Miao Peng, Jiao Jin
School of Life Sciences, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250117, PR China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, PR China; Jinan Guoke Medical Technology Development Co, Ltd, Jinan, 250103, PR China.
Biosens Bioelectron. 2023 May 15;228:115226. doi: 10.1016/j.bios.2023.115226. Epub 2023 Mar 11.
Circular RNAs (circRNAs) can act as molecular sponges of microRNA (miRNA) to form circRNA-miRNA axis, which regulates the expressions of downstream proteins. Although the mechanism has been widely reported in various bioprocesses, there is still a lack of reliable and facile way to intuitively monitor and locate the interaction between circRNA and miRNA inside living cells. In this study, multiple DNA probes are designed and loaded onto two-dimensional Cu metal-organic framework (2D Cu-MOF) nanosheets for one-step analysis of circRNA-miRNA axis. The nanosheets serve as not only powerful fluorescence quenchers but also excellent nanocarriers of abundant DNA probes for further assembly. The Probes@Cu-MOF complex can be applied to track the circRNA-miRNA axis in living cells with high sensitivity and co-localization analysis. This platform combines the transmembrane advantage of nanosheets and the signal amplification ability of localized DNA cascade assembly, so it holds great potential for understanding the biological functions of circRNA-miRNAs in cancer pathogenesis and for therapeutic applications.
环状RNA（circRNAs）可作为微小RNA（miRNA）的分子海绵，形成circRNA-miRNA轴，从而调节下游蛋白质的表达。尽管该机制已在各种生物过程中得到广泛报道，但仍然缺乏一种可靠且简便的方法来直观地监测和定位活细胞内circRNA与miRNA之间的相互作用。在本研究中，设计了多个DNA探针并将其负载到二维铜金属有机框架（2D Cu-MOF）纳米片上，用于对circRNA-miRNA轴进行一步分析。这些纳米片不仅是强大的荧光猝灭剂，也是用于进一步组装的丰富DNA探针的优秀纳米载体。Probes@Cu-MOF复合物可用于以高灵敏度和共定位分析追踪活细胞中的circRNA-miRNA轴。该平台结合了纳米片的跨膜优势和局部DNA级联组装的信号放大能力，因此在理解circRNA-miRNAs在癌症发病机制中的生物学功能以及治疗应用方面具有巨大潜力。
