Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestr. 9, 91054, Erlangen, Germany.
Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestr. 9, 91054, Erlangen, Germany; Fraunhofer Institute for Process Engineering and Packaging IVV, Giggenhauser Str. 35, 85354, Freising, Germany.
Anal Chim Acta. 2023 Apr 29;1252:341067. doi: 10.1016/j.aca.2023.341067. Epub 2023 Mar 7.
Body odor is an important aspect in interpersonal communication. For psychological and chemical research on body odors, suitable procedures for sampling and application, and analysis of body odors are essential. In this study, different textile fabrics (polyester, cotton, and Gazin gauze) were comparatively evaluated in view of their potential suitability for body odor sampling by determining recovery rates of selected body odor substances. In addition, the impact of sample preparation and storage conditions on the recovery rates was determined.
The recovery rates of dimethyl disulfide, (E)-non-2-enal, 5α-androst-16-en-3-one, 6-methylhept-5-en-2-one, heptanal and 3-sulfanylhexan-1-ol were determined under different conditions of storage (storage for 30 min at room temperature or storage for 30 min at room temperature followed by freezing at - 80 °C for 4 weeks) and sample work-up (solvent extraction with and without solvent-assisted flavour evaporation, in the following: SAFE). SAFE led to overall lower recovery rates with a significant effect for (E)-non-2-enal and 5α-androst-16-en-3-one. Nevertheless, the results showed that SAFE can be an essential step when working with a complex matrix. When comparing the different fabrics, except for (E)-non-2-enal no difference between the recovery rates obtained for cotton and polyester became evident. Gazin gauze showed lower recovery rates for all compounds. Finally, our results showed that the here investigated target compounds are stable during storage for four weeks at - 80 °C.
The results show on the one hand that the here investigated compounds were stable during storage and that the fiber type had limited influence on overall recovery rates. On the other hand, they highlight the limitations of using textile materials for sampling of volatile substances, especially with regard to low recovery rates for certain substances and the necessity of material pre-treatment or distillation steps for enabling GC-MS analysis after solvent extraction.
体臭是人际交流中的一个重要方面。对于体臭的心理和化学研究，合适的采样和应用程序以及体臭分析是必不可少的。在这项研究中，通过确定选定体臭物质的回收率，比较评估了不同的纺织面料（聚酯，棉和 Gazin 纱布）在体臭采样方面的潜在适用性。此外，还确定了样品制备和存储条件对回收率的影响。
在不同的存储条件下（在室温下储存 30 分钟或在室温下储存 30 分钟后再在-80°C下冷冻 4 周）和样品处理（有和没有溶剂辅助风味蒸发的溶剂萃取，如下所示：SAFE）下，测定了二甲基二硫，（E）-壬烯醛，5α-雄甾-16-烯-3-酮，6-甲基庚-5-烯-2-酮，庚醛和 3-巯基己烷-1-醇的回收率。SAFE 导致整体回收率较低，对（E）-壬烯醛和 5α-雄甾-16-烯-3-酮有显著影响。尽管如此，结果表明，当处理复杂基质时，SAFE 可能是必不可少的步骤。当比较不同的织物时，除了（E）-壬烯醛之外，棉和聚酯的回收率之间没有明显差异。Gazin 纱布对所有化合物的回收率均较低。最后，我们的结果表明，在所研究的目标化合物在-80°C下储存四周时是稳定的。
一方面，结果表明，在所研究的化合物在储存过程中是稳定的，并且纤维类型对整体回收率的影响有限。另一方面，它们突出了使用纺织材料采样挥发性物质的局限性，尤其是对于某些物质的回收率低以及在溶剂萃取后进行 GC-MS 分析之前需要对材料进行预处理或蒸馏步骤的必要性。
