使用恒河猴和食蟹猴检测登革病毒的神经毒力

Neurovirulence detection of dengue virus using rhesus and cynomolgus monkeys.

作者信息

Angsubhakorn S, Moe J B, Marchette N J, Latendresse J R, Palumbo N E, Yoksan S, Bhamarapravati N

机构信息

Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.

出版信息

J Virol Methods. 1987 Oct;18(1):13-24. doi: 10.1016/0166-0934(87)90106-6.

DOI:10.1016/0166-0934(87)90106-6

PMID:3693516

Abstract

The results of a comparative study of neurovirulence of dengue type 1 virus in two species of Old World monkeys, viz. rhesus monkeys (Macaca mulatta) and cynomolgus monkeys (Macaca fascicularis) are reported. In the present study, parental dengue type 1 (16007) and its vaccine viruses were tested by intrathalamic, intramuscular and intraspinal injections in these two species of monkey. Both species of monkeys inoculated with parental dengue type 1 virus developed neurovirulence-type lesions which were graded as minimal (V-1) and occasionally mild (V-2, in cynomolgus monkeys) in severity. The antibody response to either parental or vaccine virus was slightly less in rhesus monkeys than in cynomolgus inoculated with these strains. This comparative study possibly establishes the cynomolgus monkey as a suitable test model to replace the rhesus monkey for neurovirulence testing of dengue-1 vaccine intended for use in humans.

摘要

报告了对1型登革病毒在两种旧大陆猴，即恒河猴（猕猴）和食蟹猴（食蟹猕猴）中的神经毒力进行比较研究的结果。在本研究中，通过在这两种猴中进行丘脑内、肌肉内和脊髓内注射，对亲代1型登革病毒（16007）及其疫苗病毒进行了测试。接种亲代1型登革病毒的两种猴均出现了神经毒力型病变，病变严重程度分级为轻度（V-1），在食蟹猴中偶尔为中度（V-2）。与接种这些毒株的食蟹猴相比，恒河猴对亲代或疫苗病毒的抗体反应略低。这项比较研究可能确立了食蟹猴作为一种合适的试验模型，以取代恒河猴用于对人类使用的1型登革疫苗进行神经毒力测试。