Istanbul University, Istanbul Faculty of Medicine, Department of Medical Biology, Istanbul, Türkiye.
University of Health Sciences, Bakırköy Sadi Konuk Education and Research Hospital, Department of Gynecologic Oncology, Istanbul, Türkiye.
Cell Biochem Biophys. 2024 Dec;82(4):3345-3350. doi: 10.1007/s12013-024-01416-4. Epub 2024 Jul 17.
Mesenchymal stem cells (MSCs) are promising for clinical studies owing to their self-renewal, multipotency, trophic, and immunomodulatory properties. This study aimed to investigate the cytokine levels of human umbilical cord blood (CB) and Wharton's Jelly-(WJ) derived MSCs relevant to immune modulation on different passage levels in vitro. Umbilical CB MSCs were isolated using the ficoll-paque gradient method, and WJ-MSCs were isolated by the explant method. After isolation, the MSCs were characterized using flow cytometry. The supernatant cytokine levels (interferon-gamma (IFN-γ), interleukin 4 (IL-4), interleukin 17 (IL-17)) of MSCs at each passage were evaluated using the ELISA assay. MSCs exhibited different cytokine levels with each passage number. In WJ-MSC culture supernatants, IL-17 levels significantly increased at P4 and P5 compared to the first passage (p < 0.005), while the other passages showed a decrease. IFN-γ levels increased at passage P1 and P4 and decreased at other passages (p < 0.005). IL-4 levels significantly increased only at passage P3 (p < 0.005). In CB-MSC culture supernatants, IL-17 and IL-4 cytokines decreased compared to P0, while IFN-γ cytokine increased from P0 (p < 0.005). The changing ratio of cytokine levelsfor both CB-MSCs and WJ-MSCs were similarly maintained from early to late passages. More research is needed to understand the immunomodulatory functions of MSCs.
间充质干细胞（MSCs）因其自我更新、多能性、营养和免疫调节特性而备受临床研究关注。本研究旨在研究人脐血（CB）和 Wharton 胶（WJ）来源的间充质干细胞在体外不同传代水平的免疫调节相关细胞因子水平。使用 ficoll-paque 梯度法分离脐 CB-MSCs，通过组织块法分离 WJ-MSCs。分离后，使用流式细胞术对 MSC 进行鉴定。使用 ELISA 测定法评估 MSC 各代上清液中的细胞因子水平（干扰素-γ（IFN-γ）、白细胞介素 4（IL-4）、白细胞介素 17（IL-17））。MSCs 的细胞因子水平随传代次数的不同而不同。在 WJ-MSC 培养上清液中，与第一代相比，第 4 代和第 5 代的 IL-17 水平显著升高（p<0.005），而其他代则降低。IFN-γ水平在第 1 代和第 4 代增加，而在其他代则降低（p<0.005）。仅在第 3 代时 IL-4 水平显著增加（p<0.005）。在 CB-MSC 培养上清液中，与 P0 相比，IL-17 和 IL-4 细胞因子减少，而 IFN-γ 细胞因子从 P0 开始增加（p<0.005）。CB-MSCs 和 WJ-MSCs 的细胞因子水平变化比例从早期到晚期传代相似。需要进一步研究以了解 MSCs 的免疫调节功能。
