School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.
School of Chemistry and Forensics, University of Kent, Canterbury, Kent CT2 7NJ, UK.
Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27.
We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from . These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing proteins from bacteria. The release of vesicle-packaged proteins supports isolation from the culture and allows long-term storage of active protein. This technology results in high yields of vesicle-packaged, functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.
我们描述了一种创新系统，能够从. 中以膜结合囊泡的形式输出多种重组蛋白。这些重组囊泡将蛋白质分隔在一个微环境中，从而使原本难以生产的不溶性、毒性或含有二硫键的蛋白质能够在细菌中表达。囊泡包裹的蛋白质的释放支持从培养物中分离，并允许活性蛋白质的长期储存。这项技术可实现囊泡包裹的、功能性蛋白质的高产率，从而为从发现科学到应用生物技术和医学的广泛应用提供高效的下游处理。
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