Department of Pharmacology, School of Allied Health Sciences, Malla Reddy University, Hyderabad, Telangana, India.
Department of Pharmacology, School of Pharmacy, KPJ Healthcare University College, Nilai, Malaysia.
J Med Life. 2023 Feb;16(2):307-316. doi: 10.25122/jml-2022-0151.
Food supplements are used to improve cognitive functions in age-related dementia. This study was designed to determine the leaves' effect on Alloxan-induced cognitive impairment in diabetic rats and the contents of oxidative stress biomarkers, catalase, reduced glutathione, and glutathione reductase in brain tissue homogenates. Wistar rats were divided into seven groups (six rats per group). Group I received saline water (1 ml, p.o.), Diabetes was induced in Groups II-VII with Alloxan (120 mg/kg/p.o). Group III was provided with Donepezil HCl (2.5 mg/kg/p.o.), Group IV, V, VI, and VII with ethanol extract (200 and 400 mg/kg/p.o.) and aqueous extract (200 and 400 mg/kg/p.o.), respectively, for 30 days. Behavior, acetylcholinesterase (AChE) activity, oxidative stress status, and histopathological features were determined in the hippocampus and cerebral cortex. Administration of ethanolic and aqueous extracts significantly (P<0.05, P<0.001) increased the number of holes crossed by rats from one chamber to another. There was an increase in the (1) latency to reach the solid platform, (2) number of squares traveled by rats on the 30 day, and (3) percentage of spontaneous alternation behavior compared to the control group. Administration for successive days markedly decreased AChE activity (P<0.05), decreased TBARS level, and increased catalase, GSH, and GR levels. Murayya koenigii could be a promising food supplement for people with dementia. However, more research into sub-chronic toxicity and pharmacokinetic and pharmacodynamics interactions is essential.
膳食补充剂用于改善与年龄相关的痴呆症中的认知功能。本研究旨在确定叶对糖尿病大鼠中丙烯醛诱导的认知障碍的影响，以及脑组织匀浆中氧化应激生物标志物过氧化氢酶、还原型谷胱甘肽和谷胱甘肽还原酶的含量。Wistar 大鼠分为七组（每组六只大鼠）。第 I 组给予生理盐水（1 ml，po）。第 II-VII 组用丙烯醛（120 mg/kg，po）诱导糖尿病。第 III 组给予盐酸多奈哌齐（2.5 mg/kg，po），第 IV、V、VI 和 VII 组分别给予乙醇提取物（200 和 400 mg/kg，po）和水提取物（200 和 400 mg/kg，po），共 30 天。在海马和大脑皮层中测定行为、乙酰胆碱酯酶（AChE）活性、氧化应激状态和组织病理学特征。乙醇和水提取物的给药显著（P<0.05，P<0.001）增加了大鼠从一个室到另一个室的孔数。（1）到达固体平台的潜伏期、（2）大鼠在第 30 天穿过的正方形数量以及（3）自发交替行为的百分比均有所增加与对照组相比。连续给药显著降低 AChE 活性（P<0.05），降低 TBARS 水平，增加过氧化氢酶、GSH 和 GR 水平。Murayya koenigii 可能是痴呆症患者有希望的膳食补充剂。然而，需要进一步研究亚慢性毒性以及药代动力学和药效学相互作用。
