Mazière C, Mazière J C, Mora L, Polonovski J
Laboratoire de Biochimie, Faculté de Médecine Saint-Antoine, Paris, France.
J Biochem Biophys Methods. 1987 Aug;14(5):267-72. doi: 10.1016/0165-022x(87)90052-2.
A method is described where cell suspension obtained by scraping of monolayers was directly applied on silica gel plates. Extraction and separation of different lipid classes were simultaneously obtained during chromatography. In the range of validity of the method (no more than 80 micrograms of cellular protein tested for neutral lipids and 30 micrograms for phospholipids), this technique allows rapid lipid analysis of small samples of cultured cells, bypassing all the time- and solvent-consuming extraction and evaporation steps. The method appears to be also suitable for measurement of enzymes of lipid metabolism such as acyl coenzyme A-cholesterol-acyltransferase.
本文描述了一种方法，即通过刮擦单层细胞获得的细胞悬液直接应用于硅胶板上。在色谱过程中可同时实现不同脂质类别的提取和分离。在该方法的有效范围内（测试中性脂质时细胞蛋白不超过80微克，测试磷脂时不超过30微克），该技术无需经过耗时且耗溶剂的提取和蒸发步骤，就能对培养细胞的小样本进行快速脂质分析。该方法似乎也适用于脂质代谢酶如酰基辅酶A - 胆固醇 - 酰基转移酶的测定。
