利用国产数字全息图像系统评估人类精子头部形态的初步研究
A Preliminary Study on the Evaluation of Human Sperm Head Morphology with a Domestic Digital Holographic Image System.
作者信息
Zhu Hong, Zhu Yong, Sun Can, Jiang Feng
机构信息
Department of Urology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011 China.
Human Sperm Bank of Fudan University, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011 China.
出版信息
Phenomics. 2022 Feb 26;2(2):130-135. doi: 10.1007/s43657-022-00046-1. eCollection 2022 Apr.
The head of sperm was imaged with domestic digital holographic microscopy (DHM), and then the quantitative three-dimensional size information of normal sperm and teratozoospermic sperm was compared and analyzed. DHM sperm imaging and repeated quantitative evaluation were used to determine the morphology of the sperm head in two patients with teratozoospermia and four volunteers with normal semen parameters. Sixty and 139 sperm of teratozoospermia patients and normal people were photographed by digital hologram, respectively. The differences in head height and width were compared and statistically analyzed. The sperm head height of the teratozoospermia group was 3.06 ± 1.66 μm, which was significantly lower than that of the normal sperm group (4.54 ± 1.60 μm, < 0.01), but there was no significant difference in the head width between the two groups. Compared with the traditional two-dimensional optical microscope observation method, the DHM system can provide three-dimensional quantitative information for the sperm head and thus may help in the comprehensive clinical evaluation of the sperm head structure.
使用国产数字全息显微镜（DHM）对精子头部进行成像，然后比较并分析正常精子和畸形精子症患者精子的三维定量尺寸信息。采用DHM精子成像和重复定量评估来确定两名畸形精子症患者和四名精液参数正常的志愿者精子头部的形态。分别对畸形精子症患者和正常人的60个及139个精子进行数字全息摄影。比较并统计分析头部高度和宽度的差异。畸形精子症组精子头部高度为3.06±1.66μm，显著低于正常精子组（4.54±1.60μm，<0.01），但两组之间头部宽度无显著差异。与传统二维光学显微镜观察方法相比，DHM系统可为精子头部提供三维定量信息，从而可能有助于精子头部结构的临床综合评估。
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