School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USA.
School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USA.
Environ Toxicol Pharmacol. 2023 Apr;99:104113. doi: 10.1016/j.etap.2023.104113. Epub 2023 Mar 20.
Growth hormone-releasing hormone (GHRH) regulates the synthesis of growth hormone from the anterior pituitary gland, and it is involved in inflammatory responses. On the other hand, GHRH antagonists (GHRHAnt) exhibit the opposite effects, resulting in endothelial barrier enhancement. Exposure to hydrochloric acid (HCL) is associated with acute and chronic lung injury. In this study, we investigate the effects of GHRHAnt in HCL-induced endothelial barrier dysfunction, utilizing commercially available bovine pulmonary artery endothelial cells (BPAEC). Cell viability was measured by utilizing 3-(4,5-dimethylthiazol2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay. Moreover, fluorescein isothiocyanate (FITC)-dextran was used to assess barrier function. Our observations suggest that GHRHAnt exert protective effects against HCL-induced endothelial breakdown, since those peptides counteract HCL-triggered paracellular hyperpermeability. Based on those findings, we propose that GHRHAnt represent a new therapeutic approach towards HCL-induced endothelial injury.
生长激素释放激素 (GHRH) 调节垂体前叶生长激素的合成，参与炎症反应。另一方面，GHRH 拮抗剂 (GHRHAnt) 表现出相反的作用，导致内皮屏障增强。接触盐酸 (HCL) 与急性和慢性肺损伤有关。在这项研究中，我们使用市售的牛肺动脉内皮细胞 (BPAEC) 研究了 GHRHAnt 在 HCL 诱导的内皮屏障功能障碍中的作用。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐 (MTT) 测定法测量细胞活力。此外，还使用荧光素异硫氰酸酯 (FITC)-葡聚糖评估屏障功能。我们的观察表明，GHRHAnt 对 HCL 诱导的内皮破裂具有保护作用，因为这些肽可以对抗 HCL 触发的细胞旁通透性增加。基于这些发现，我们提出 GHRHAnt 是治疗 HCL 诱导的内皮损伤的一种新方法。
