Widyarman Armelia Sari, Halim Louise Anastasya, Irma Heidi Amanda, Richi Mario, Rizal Muhammad Ihsan
Department Head of Microbiology, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.
Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.
Saudi Dent J. 2023 Feb;35(2):154-164. doi: 10.1016/j.sdentj.2023.01.002. Epub 2023 Jan 5.
Despite the use of common irrigating solution with antimicrobial properties, failed root canal treatment remains a significant problem in endodontics. In the present study, we examined the efficacy of reuterin derived from probiotic bacteria, on the biofilms of major endodontic pathogens using ex vivo model of root canal infections.
Biofilms of major endodontic pathogens namely , , and were formed on root canals of 60 human premolar tooth samples accordingly a standard protocol. Thereafter, teeth were treated with either 2.5 % NaOCl (positive control), various concentrations of reuterin (test-group) or sterilized-distilled water (negative control) in a time-dependent assay. The efficacy of irrigation was evaluated by a time-dependent assay at 5 min and 30 min after irrigation by colony-forming units assay. The findings were further confirmed by species-specific real-time PCR. Data were statistically analysed using one way ANOVA with a significance level of P < 0.05.
Reuterin isolated from was effective against , , , and biofilms, with a concentration of 100 µg/mL being the most effective compared to the negative control (P < 0.05) and also showed similar efficacy when compared with NaOCl
Reuterin isolated from has ability to inhibit in vitro and ex-vivo biofilms of endodontic pathogens, namely and Reuterin has potential as a root canal irrigating solution due to its antibiofilm activity. Further research is warranted to determine the potential of probiotic bacteriotherapy in root canal systems.
尽管使用了具有抗菌特性的常用冲洗液，但根管治疗失败仍是牙髓病学中的一个重大问题。在本研究中，我们使用根管感染的体外模型，研究了源自益生菌的罗伊氏菌素对主要牙髓病病原体生物膜的疗效。
按照标准方案，在60颗人类前磨牙样本的根管上形成主要牙髓病病原体即[具体病原体1]、[具体病原体2]、[具体病原体3]和[具体病原体4]的生物膜。此后，在时间依赖性试验中，用2.5%次氯酸钠（阳性对照）、不同浓度的罗伊氏菌素（试验组）或灭菌蒸馏水（阴性对照）对牙齿进行处理。通过菌落形成单位测定法，在冲洗后5分钟和30分钟进行时间依赖性试验，评估冲洗效果。通过种特异性实时PCR进一步证实研究结果。使用单因素方差分析对数据进行统计学分析，显著性水平为P < 0.05。
从[具体来源]分离出的罗伊氏菌素对[具体病原体1]、[具体病原体2]、[具体病原体3]和[具体病原体4]生物膜有效，与阴性对照相比，浓度为100μg/mL时最有效（P < 0.05），与次氯酸钠相比也显示出相似的疗效。
从[具体来源]分离出的罗伊氏菌素有能力在体外和体内抑制牙髓病病原体即[具体病原体1]和[具体病原体2]的生物膜。由于其抗生物膜活性，罗伊氏菌素有潜力作为根管冲洗液。有必要进一步研究以确定益生菌细菌疗法在根管系统中的潜力。
